Metcalf J F, Kaufman H E
Am J Ophthalmol. 1976 Dec;82(6):827-34. doi: 10.1016/0002-9394(76)90057-x.
Immunofluorescence, histological, and electron microscopic observations were made on rabbit corneas from animals with experimentally induced stromal keratitis following intracorneal injection with the RE strain of herpes simplex virus. Electron microscopic observations were also made on human corneas obtained from patients with a history of herpetic stromal disease. Viral antigens were demonstrated by immunofluorescence in keratocytes of rabbit corneas with herpetic stromal keratitis. Electron microscopic observations and viral culture failed to reveal the presence of viral particles in these tissues. Lymphocytes, a major infiltrating cell type found in both the rabbit and human corneas, were often found in intimate contact with degenerating keratocytes.
对经角膜内注射单纯疱疹病毒RE株后实验性诱发基质性角膜炎的家兔角膜进行了免疫荧光、组织学和电子显微镜观察。还对有疱疹性基质疾病病史患者的人角膜进行了电子显微镜观察。在患有疱疹性基质性角膜炎的家兔角膜的角膜细胞中，通过免疫荧光证实了病毒抗原的存在。电子显微镜观察和病毒培养未能揭示这些组织中存在病毒颗粒。淋巴细胞是在家兔和人角膜中均发现的主要浸润细胞类型，常发现其与变性的角膜细胞紧密接触。
