Chong K T, Mims C A
J Gen Virol. 1981 Dec;57(Pt 2):415-9. doi: 10.1099/0022-1317-57-2-415.
Murine cytomegalovirus (MCMV) preparations from mouse embryo fibroblasts and from infected salivary glands were purified on potassium tartrate density gradients and examined by electron microscopy. The cell culture virus contained multicapsid enveloped virions which are ruptured easily and account for the excess number of free capsids in these preparations. Salivary gland virus consisted of single-capsid enveloped virus and equal numbers of free capsids. Particle types were imperfectly separated on density gradients, but successful separation was achieved by filtration through 220 and 450 nm Millipore membrane filters. Naked capsids were not infectious and could not be rendered infectious by centrifugal adsorption, showing that centrifugal enhancement of MCMV infectivity is not mediated through this mechanism. Although present in excess number in (avirulent) cell culture virus preparations, naked capsids did not interfere with the action of (virulent) salivary gland virus in newborn mice.
从小鼠胚胎成纤维细胞和感染的唾液腺中获得的鼠巨细胞病毒（MCMV）制剂，在酒石酸钾密度梯度上进行纯化，并通过电子显微镜检查。细胞培养病毒包含多衣壳包膜病毒粒子，这些粒子很容易破裂，这就解释了这些制剂中游离衣壳数量过多的原因。唾液腺病毒由单衣壳包膜病毒和等量的游离衣壳组成。在密度梯度上粒子类型分离不完全，但通过220和450纳米的密理博膜过滤器过滤可实现成功分离。裸露的衣壳没有传染性，通过离心吸附也不能使其具有传染性，这表明MCMV感染性的离心增强不是通过这种机制介导的。尽管在（无毒的）细胞培养病毒制剂中裸露衣壳数量过多，但它们并不干扰（有毒的）唾液腺病毒在新生小鼠中的作用。
