细胞内水泡性口炎病毒前导RNA存在于核衣壳结构中。
Intracellular vesicular stomatitis virus leader RNAs are found in nucleocapsid structures.
作者信息
Blumberg B M, Kolakofsky D
出版信息
J Virol. 1981 Nov;40(2):568-76. doi: 10.1128/JVI.40.2.568-576.1981.
Abstract
Previous studies demonstrated that cytoplasmic extracts of cells infected with vesicular stomatitis virus contain plus-strand leader RNAs which sediment at 18S on sucrose gradients as a complex with viral N protein. The work presented in this paper demonstrated that these 18S complexes were stable on CsCl density gradients, banding at a buoyant density near that of genome nucleocapsids, and exhibited a morphology in an electron microscope similar to the disk structures found in virus genome nucleocapsids. Minus-strand leader RNAs were also found in 18S complexes on sucrose gradients. Quantitation of intracellular leader RNA suggested that, late in infection, approximately three-quarters of total intracellular leader RNA was encapsidated.
摘要
先前的研究表明，感染水疱性口炎病毒的细胞的细胞质提取物含有正链前导RNA，其在蔗糖梯度上以18S沉降，与病毒N蛋白形成复合物。本文所展示的研究表明，这些18S复合物在CsCl密度梯度上是稳定的，在接近基因组核衣壳的浮力密度处形成条带，并且在电子显微镜下呈现出与病毒基因组核衣壳中发现的盘状结构相似的形态。在蔗糖梯度上的18S复合物中也发现了负链前导RNA。细胞内前导RNA的定量分析表明，在感染后期，细胞内总前导RNA中约四分之三被包装起来。
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