Kimura T, Sawada Y, Shinawawa M, Shimizu Y, Shiroki K, Shimojo H, Sugisaki H, Takanami M, Uemizu Y, Fujinaga K
Nucleic Acids Res. 1981 Dec 11;9(23):6571-89. doi: 10.1093/nar/9.23.6571.
The nucleotide sequence of the entire transforming early region of E1b of the highly oncogenic adenovirus type 12 (Ad12) DNA has been determined. The total sequence (3860 base pairs) encompasses the entire transforming early region E1 of Ad12 DNA. From the sequence for the E1b region of Ad12, and the transcription map of the E1b region (1, 2, 3, and this paper) the structure and gene organization of the early region E1b of Ad12 DNA were analyzed and compared with those of the E1b region in the non-oncogenic Ad5 DNA (4, 5). Most of the sequences in the E1b region of Ad12 was highly homologous to that of Ad5. It is predicted that the Ad12 region E1b codes for polypeptides of 53.9, 19.1, and 8.9 kd. This situation is identical with that of the Ad5 region E1b which codes for polypeptides of 54.9, 20.6, and 8.3 kd. The function of these predicted polypeptides encoded by the E1b regions in cell transformation is discussed.
已确定高致癌性12型腺病毒（Ad12）DNA的E1b整个转化早期区域的核苷酸序列。总序列（3860个碱基对）涵盖了Ad12 DNA的整个转化早期区域E1。根据Ad12的E1b区域序列以及E1b区域的转录图谱（参考文献1、2、3以及本文），分析了Ad12 DNA早期区域E1b的结构和基因组织，并与非致癌性Ad5 DNA的E1b区域进行了比较（参考文献4、5）。Ad12的E1b区域中的大多数序列与Ad5的高度同源。预计Ad12区域E1b编码53.9、19.1和8.9千道尔顿的多肽。这种情况与Ad5区域E1b相同，后者编码54.9、20.6和8.3千道尔顿的多肽。讨论了由E1b区域编码的这些预测多肽在细胞转化中的功能。
