Brown A H, Niles N R, Braimbridge M V, Austen W G
J Cardiovasc Surg (Torino). 1978 Jan-Feb;19(1):29-48.
We have compared results of isovolumic ventricular function studies and results of histochemical and birefringence examinations of biopsy specimens from 80 isolated canine hearts both before and after three hours of preservation utilizing several variables. The direct and derived mechanical tests and histochemical and birefringence assessments of the myocardium were correlated by multiple regression analysis. Our results suggest that initial ventricular health may be indicated by acid hematein staining of free phospholipids and by the response of myocardial birefringence to adenosine triphosphate and calcium, while succinic dehydrogenase staining and birefringence without adenosine-triphosphate treatment are more useful in showing ventricular deterioration during operation.
我们利用多个变量，比较了80颗离体犬心在保存三小时前后等容心室功能研究的结果，以及活检标本的组织化学和双折射检查结果。通过多元回归分析，将心肌的直接和派生机械测试以及组织化学和双折射评估进行了关联。我们的结果表明，游离磷脂的酸性苏木精染色以及心肌双折射对三磷酸腺苷和钙的反应可能表明初始心室健康，而琥珀酸脱氢酶染色和未经三磷酸腺苷处理的双折射在显示手术期间心室恶化方面更有用。
