de Vries H, Haima P, Brinker M, de Jonge J C
FEBS Lett. 1985 Jan 7;179(2):337-42. doi: 10.1016/0014-5793(85)80547-0.
We have sequenced a 682 bp fragment of Neurospora crassa mitochondrial DNA to complete the sequence between the gene for cytochrome b and the unassigned reading frame, URF U. The sequence contains a gene for a cysteine tRNA. The 5' end of the 6 kb polycistronic transcript of cytochrome c oxidase subunit 1 is immediately downstream from this tRNA. This shows that also in fungal mitochondria tRNAs can be used as processing signals, whereas palindromic sequences containing double Pst I sites, also present in this region, are not used for processing.
我们对粗糙脉孢菌线粒体DNA的一段682 bp片段进行了测序，以完成细胞色素b基因与未分配阅读框URF U之间的序列。该序列包含一个半胱氨酸tRNA基因。细胞色素c氧化酶亚基1的6 kb多顺反子转录本的5'端紧邻该tRNA的下游。这表明在真菌线粒体中，tRNA也可用作加工信号，而该区域中同样存在的含有两个Pst I位点的回文序列则不用于加工。
