Diegelmann R F, Peterkofsky B
Proc Natl Acad Sci U S A. 1972 Apr;69(4):892-6. doi: 10.1073/pnas.69.4.892.
Collagen synthesis in chick-embryo frontal bone and 3T3 fibroblasts from mice was measured by incorporation in vitro of [(14)C]proline into collagenase-digestible material. About 15-25% of the collagen synthesized by the frontal bone in 60 min, and 60% of that synthesized by the fibroblasts in 2 hr, was found to be soluble in the culture medium. The microtubular disruptive drugs colchicine and vinblastine, at 10 muM, inhibited collagen secretion in both systems almost completely. Formation of collagen hydroxyproline from proline was not inhibited by these drugs. Cytochalasin B, which impairs microfilament function, had no effect on collagen secretion. Our results support the theory that collagen is transported in vesicles to the cell membrane, where it is secreted. This conclusion is based on the similarity of the collagen-secreting system to other systems in which the movement of secretory vesicles or storage granules is inhibited by microtubule disruption.
通过体外将[（14）C]脯氨酸掺入胶原酶可消化物质中，测定鸡胚额骨和小鼠3T3成纤维细胞中的胶原蛋白合成。发现额骨在60分钟内合成的胶原蛋白约15 - 25%，以及成纤维细胞在2小时内合成的胶原蛋白的60%，可溶于培养基。10μM的微管破坏药物秋水仙碱和长春碱几乎完全抑制了两个系统中的胶原蛋白分泌。这些药物并未抑制脯氨酸形成胶原羟脯氨酸。损害微丝功能的细胞松弛素B对胶原蛋白分泌没有影响。我们的结果支持这样一种理论，即胶原蛋白通过囊泡运输到细胞膜并在那里分泌。这一结论基于胶原蛋白分泌系统与其他系统的相似性，在其他系统中，分泌囊泡或储存颗粒的移动会被微管破坏所抑制。
