Stout R W
Diabetologia. 1982 Jan;22(1):51-5. doi: 10.1007/BF00253870.
The effect of dibutyryl cyclic AMP on DNA synthesis was studied in cultured human umbilical endothelial cells and rat aortic smooth muscle cells. Dibutyryl cyclic AMP (2 X 10(-4) mol/l) inhibited DNA synthesis in both arterial cell types when they were grown in medium supplemented with whole serum or with platelet poor serum, but had no effect in the absence of serum. An effect was seen one hour after the addition of the nucleotide, and the threshold concentration was between 2 X 10(-6) and 2 X 10(-5) mol/l. These results may have relevance to the interaction of platelets and insulin with the arterial wall in the development of atherosclerosis in diabetes.
在培养的人脐静脉内皮细胞和大鼠主动脉平滑肌细胞中研究了二丁酰环磷腺苷对DNA合成的影响。当在补充有全血清或血小板贫乏血清的培养基中培养时，二丁酰环磷腺苷（2×10⁻⁴mol/L）抑制这两种动脉细胞类型中的DNA合成，但在无血清时无作用。加入该核苷酸1小时后可见效应，阈值浓度在2×10⁻⁶至2×10⁻⁵mol/L之间。这些结果可能与糖尿病动脉粥样硬化发生过程中血小板和胰岛素与动脉壁的相互作用有关。
