急性腺病毒感染的酶联免疫吸附测定

Enzyme-linked immunosorbent assay for acute adenovirus infection.

作者信息

Roggendorf M, Wigand R, Deinhardt F, Frösner G G

出版信息

J Virol Methods. 1982 Feb;4(1):27-35. doi: 10.1016/0166-0934(82)90051-9.

Abstract

An enzyme-linked immunosorbent assay (ELISA) is described for demonstrating antibodies to the hexone antigen of adenoviruses. The antigen-coated, flat-bottomed microtiter plates are incubated sequentially with dilutions of patients' sera (2 h at 37 degrees C) and peroxidase-coupled anti-human IgG (2 h at 37 degrees C). After a final washing, orthophenylenediamine is added to the plates, and the absorbance (A) measured 30 min later. The ELISA was found to be a hundred-fold more sensitive than complement fixation. An evaluation methods for determining antibody concentration is described which correlates the absorbance of sera diluted 10(-3) to the absorbance of a reference serum containing an arbitrary value (100) of antibody. This methods avoids titration of sera and day-to-day assay variations by different background reactions. A significant increase in antibody concentration of acute-phase serum over that of convalescent phase serum is observed. The ability to test sera in a single dilution and the automatic reading of results and their evaluation by computer make this assay suitable for diagnostic laboratories.

摘要

本文描述了一种用于检测腺病毒六邻体抗原抗体的酶联免疫吸附测定（ELISA）方法。将包被有抗原的平底微量滴定板依次与患者血清稀释液（37℃孵育2小时）和过氧化物酶偶联的抗人IgG（37℃孵育2小时）孵育。最后洗涤后，向板中加入邻苯二胺，并在30分钟后测量吸光度（A）。发现ELISA的灵敏度比补体结合试验高100倍。本文描述了一种测定抗体浓度的评估方法，该方法将稀释10^(-3)的血清吸光度与含有任意值（100）抗体的参考血清吸光度相关联。该方法避免了血清滴定以及不同背景反应导致的日常检测差异。观察到急性期血清抗体浓度比恢复期血清有显著增加。能够以单一稀释度检测血清，以及结果的自动读取和通过计算机进行评估，使得该检测方法适用于诊断实验室。