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[腺病毒的实验室操作。IX. 用对流免疫电泳法测定六邻体抗体]

[Laboratory procedures with adenoviruses. IX. Hexon antibody determination by countercurrent immunoelectrophoresis].

作者信息

Bohn K A, Wigand R

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1983 Sep;255(2-3):196-201.

PMID:6316690
Abstract

101 paired sera from patients with proved adenovirus infections (mostly conjunctivitis) were tested quantitatively by counter immunoelectrophoresis, with hexon of adenovirus 5 as a genus-specific antigen, in comparison with complement-fixation. Both methods were equally sensitive to demonstrate antibodies; also the titers showed a marked correlation. The number of titer rises, however, was higher in counter immunoelectrophoresis; the titer rises mostly occurred around the 10th day after onset of disease. No differences between patients infected with different adenovirus types were found.

摘要

采用对流免疫电泳法,以腺病毒5型六邻体作为属特异性抗原,对101份已证实为腺病毒感染(主要为结膜炎)患者的配对血清进行了定量检测,并与补体结合试验作比较。两种方法在检测抗体方面同样敏感;滴度也显示出显著相关性。然而,对流免疫电泳中滴度升高的数量更多;滴度升高大多发生在疾病发作后第10天左右。未发现感染不同腺病毒类型的患者之间存在差异。

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