RSITE：一种预测限制性内切酶识别序列的计算机程序。
RSITE: a computer program to predict the recognition sequence of a restriction enzyme.
作者信息
Tolstoshev C M, Blakesley R W
出版信息
Nucleic Acids Res. 1982 Jan 11;10(1):1-17. doi: 10.1093/nar/10.1.1.
Abstract
A computer program (RSITE) was developed which predicts the recognition sequence of a restriction endonuclease. The sizes of fragments experimentally determined on cleavage of a DNA of known sequence were input. Possible recognition sequences producing fragments of sizes matching those determined empirically were printed out. The program faithfully predicted the specificity of restriction enzymes of known recognition sequence and also determined the recognition sequence of a new restriction enzyme from Haemophilus influenzae GU (HinGU II).
摘要
开发了一个计算机程序（RSITE），用于预测限制性内切酶的识别序列。输入已知序列DNA切割后实验测定的片段大小。打印出能产生与经验测定大小匹配的片段的可能识别序列。该程序准确地预测了已知识别序列的限制性酶的特异性，还确定了来自流感嗜血杆菌GU（HinGU II）的一种新限制性酶的识别序列。
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本文引用的文献
1
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Gene. 1980 Nov;11(3-4):219-25. doi: 10.1016/0378-1119(80)90062-1.
2
Identification of palindromic sequences recognized by restriction endonucleases, as based on the tabularized sequencing data for seven viral and plasmid DNAs.基于七种病毒和质粒DNA的列表测序数据，鉴定限制性内切核酸酶识别的回文序列。
Gene. 1980 Sep;10(4):357-70. doi: 10.1016/0378-1119(80)90156-0.
3
A cleavage map of bacteriophage phiX174 genome.噬菌体φX174基因组的切割图谱。
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2882-6. doi: 10.1073/pnas.71.7.2882.
4
Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.猴病毒40 DNA的研究。VII。SV40基因组的切割图谱。
J Mol Biol. 1973 Aug 5;78(2):363-76. doi: 10.1016/0022-2836(73)90122-8.
5
Arthrobacter luteus restriction endonuclease cleavage map of phi chi 174 RF DNA.藤黄节杆菌对φX174环状双链DNA的限制性内切酶切割图谱。
Virology. 1975 Nov;68(1):221-33. doi: 10.1016/0042-6822(75)90163-4.
6
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Nature. 1975 Oct 2;257(5525):421-2. doi: 10.1038/257421a0.
7
The nucleotide sequence of bacteriophage phiX174.噬菌体φX174的核苷酸序列。
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