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一种用于确定限制性内切酶识别位点的计算机辅助方法。

A computer assisted method for the determination of restriction enzyme recognifion sites.

作者信息

Gingeras T R, MIlazzo J P, Roberts R J

出版信息

Nucleic Acids Res. 1978 Nov;5(11):4105-27. doi: 10.1093/nar/5.11.4105.

DOI:10.1093/nar/5.11.4105
PMID:724510
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342737/
Abstract

A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has been tested using fragmentation patterns of 0X174 DNA produced by enzymes of both known and unknown specificity. Recognition sequences are predicted for two restriction endonucleases (BbvI and SfaNI) using this method. In addition, recognition sequences are predicted for two other new enzymes (PvuI and MstI) using another computer-assisted method.

摘要

已经开发出一种计算机程序，它有助于确定限制性内切酶识别序列。这是通过用限制性内切酶切割已知序列的DNA，并将片段化模式与计算机生成的一组模式进行比较来实现的。使用已知和未知特异性的酶产生的0X174 DNA的片段化模式对该方法的可行性进行了测试。使用该方法预测了两种限制性内切酶（BbvI和SfaNI）的识别序列。此外，使用另一种计算机辅助方法预测了另外两种新酶（PvuI和MstI）的识别序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79a0/342737/d9352bf8ed48/nar00472-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79a0/342737/d9352bf8ed48/nar00472-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79a0/342737/d9352bf8ed48/nar00472-0158-a.jpg

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Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.利用分析型琼脂糖-溴化乙锭电泳检测副流感嗜血杆菌中的两种限制性内切酶活性。
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Agarose slab-gel electrophoresis equipment.琼脂糖平板凝胶电泳设备。
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On the nature of the cytosine-methylated sequence in DNA of Bacillus brevis var. G.-B.关于短短芽孢杆菌变种G.-B. DNA中胞嘧啶甲基化序列的性质
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