溶液中自互补双链d(C-G-C-G-A-A-T-T-C-G-C-G)上相邻的dG.dC放线菌素结合位点与dA.dT纺锤菌素结合位点之间的相互作用。
Mutual interaction between adjacent dG . dC actinomycin binding sites and dA . dT netropsin binding sites on the self-complementary d(C-G-C-G-A-A-T-T-C-G-C-G) duplex in solution.
作者信息
Patel D J, Kozlowski S A, Rice J A, Broka C, Itakura K
出版信息
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7281-4. doi: 10.1073/pnas.78.12.7281.
Abstract
The Watson-Crick imino protons, the backbone phosphodiester resonances, and the antibiotic exchangeable protons have been used as markers to monitor the separate and simultaneous binding of actinomycin and netropsin to the d(C-G-C-G-A-A-T-T-C-G-C-G) self-complementary duplex in aqueous solution. We demonstrate that intercalation of actinomycin at dG(3'-5')dC sites at either end of the duplex results in a conformational perturbation at the dA . dT tetranucleotide core of the dodecanucleotide duplex. Parallel studies of the groove binding of netropsin at dA . dT sites in the interior of the duplex reveal a conformational perturbation which extends to adjacent dG . dC base pairs in the dodecanucleotide duplex. The NMR markers demonstrate that the d(C-G-C-G-A-A-T-T-C-G-C-G) duplex can accommodate actinomycin and netropsin simultaneously at adjacent dG . dC and dA . dT tetranucleotide blocks along its length with some mutual interaction between neighboring antibiotic binding sites.
摘要
沃森-克里克亚氨基质子、主链磷酸二酯共振峰以及抗生素可交换质子已被用作标记物，用于监测放线菌素和纺锤菌素在水溶液中与d（C-G-C-G-A-A-T-T-C-G-C-G）自互补双链体的分别结合及同时结合情况。我们证明，放线菌素嵌入双链体两端的dG（3'-5'）dC位点会导致十二聚体双链体dA·dT四核苷酸核心处的构象扰动。对纺锤菌素在双链体内部dA·dT位点的沟槽结合进行的平行研究揭示了一种构象扰动，该扰动延伸至十二聚体双链体中相邻的dG·dC碱基对。核磁共振标记物表明，d（C-G-C-G-A-A-T-T-C-G-C-G）双链体能够在其长度上相邻的dG·dC和dA·dT四核苷酸区段同时容纳放线菌素和纺锤菌素，且相邻抗生素结合位点之间存在一些相互作用。
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Mutual interaction between adjacent dG . dC actinomycin binding sites and dA . dT netropsin binding sites on the self-complementary d(C-G-C-G-A-A-T-T-C-G-C-G) duplex in solution.溶液中自互补双链d(C-G-C-G-A-A-T-T-C-G-C-G)上相邻的dG.dC放线菌素结合位点与dA.dT纺锤菌素结合位点之间的相互作用。
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Biochemistry. 1974 May 21;13(11):2396-402. doi: 10.1021/bi00708a025.
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The stereochemistry of actinomycin binding to DNA and its implications in molecular biology.放线菌素与DNA结合的立体化学及其在分子生物学中的意义。
Prog Nucleic Acid Res Mol Biol. 1973;13:153-90. doi: 10.1016/s0079-6603(08)60103-8.
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8
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