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通过抗DNase I分析放线菌素D和纺锤菌素与pBR322 DNA结合的序列特异性

Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.

作者信息

Lane M J, Dabrowiak J C, Vournakis J N

出版信息

Proc Natl Acad Sci U S A. 1983 Jun;80(11):3260-4. doi: 10.1073/pnas.80.11.3260.

DOI:10.1073/pnas.80.11.3260
PMID:6304702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC394020/
Abstract

A direct approach to determining the sequence specificities of equilibrium binding drugs by using the DNase protection technique is described. The method utilizes singly end-labeled restriction fragments and partial digestion of the drug fragment complex with DNase I. Microdensitometry of autoradiograms produced after electrophoretic separation of digestion products allows determination of sequences that are affected by drug binding. The feasibility of the technique for locating small ligands bound to DNA and its eventual use as a quantitative thermodynamic approach to studying ligand binding to heterogeneous DNA as a function of sequence is illustrated by using actinomycin D and Netropsin.

摘要

描述了一种通过使用DNA酶保护技术直接确定平衡结合药物序列特异性的方法。该方法利用单端标记的限制性片段以及用DNA酶I对药物-片段复合物进行部分消化。对消化产物进行电泳分离后产生的放射自显影片进行显微密度测定，可确定受药物结合影响的序列。通过使用放线菌素D和纺锤菌素，说明了该技术用于定位与DNA结合的小配体的可行性及其最终作为定量热力学方法来研究配体与异质DNA结合随序列变化的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bafb/394020/54cc1cb38249/pnas00637-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bafb/394020/54cc1cb38249/pnas00637-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bafb/394020/54cc1cb38249/pnas00637-0126-a.jpg

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Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.通过抗DNase I分析放线菌素D和纺锤菌素与pBR322 DNA结合的序列特异性
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3260-4. doi: 10.1073/pnas.80.11.3260.
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Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II).异质DNA上偏端霉素、纺锤菌素和放线菌素结合位点的图谱：甲基丙基乙二胺四乙酸铁（II）的DNA切割抑制模式
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本文引用的文献

1
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2
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.转录因子与5S核糖体RNA基因缺失突变体的结合。
Cell. 1981 Mar;23(3):665-9. doi: 10.1016/0092-8674(81)90429-3.
3
Equilibrium binding of carcinogens and antitumor antibiotics to DNA: site selectivity, cooperativity, allosterism.
通过混合物熔解揭示的序列和结构选择性核酸结合
Nucleic Acids Res. 2006 Jan 23;34(2):e14. doi: 10.1093/nar/gnj012.
4
Use of capillary electrophoresis in the study of ligand-DNA interactions.毛细管电泳在配体 - DNA相互作用研究中的应用。
Nucleic Acids Res. 1998 Jun 15;26(12):3053-8. doi: 10.1093/nar/26.12.3053.
5
Structure-affinity relationships for the binding of actinomycin D to DNA.放线菌素D与DNA结合的结构-亲和力关系。
J Comput Aided Mol Des. 1997 Mar;11(2):114-28. doi: 10.1023/a:1008018106064.
6
Daunomycin modifies the sequence-selective recognition of DNA by actinomycin.柔红霉素改变了放线菌素对DNA的序列选择性识别。
Nucleic Acids Res. 1994 Dec 11;22(24):5241-6. doi: 10.1093/nar/22.24.5241.
7
Selective inhibition of topoisomerases from Pneumocystis carinii compared with that of topoisomerases from mammalian cells.与哺乳动物细胞拓扑异构酶相比，卡氏肺孢子虫拓扑异构酶的选择性抑制作用。
Antimicrob Agents Chemother. 1994 Sep;38(9):1890-8. doi: 10.1128/AAC.38.9.1890.
8
DNA structural variations produced by actinomycin and distamycin as revealed by DNAase I footprinting.通过DNA酶I足迹法揭示的放线菌素和偏端霉素产生的DNA结构变异。
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