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以腺苷5'-[γ(S)-16O,17O,18O]三磷酸为底物时酵母己糖激酶催化的磷酰基转移的立体化学过程。

The stereochemical course of yeast hexokinase-catalysed phosphoryl transfer by using adenosine 5'[gamma(S)-16O,17O,18O]triphosphate as substrate.

作者信息

Lowe G, Potter B V

出版信息

Biochem J. 1981 Oct 1;199(1):227-33. doi: 10.1042/bj1990227.

DOI:10.1042/bj1990227
PMID:7039616
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163354/
Abstract

Adenosine 5'[gamma(S)-16O, 17O, 18O]triphosphate has been synthesized and used to determine the stereochemical course of phosphoryl transfer catalysed by yeast hexokinase. The chirality at phosphorus of the D-glucose 6-[16O,17O,18O]phosphate formed was analysed, after cyclization and methylation, by 31P n.m.r. spectroscopy. The phosphoryl transfer was found to occur with inversion of configuration, with a stereoselectivity in excess of 94%. The simplest interpretation of this result is that the phosphoryl group is transferred between substrates in the enzyme-substrate ternary complex by an 'in line' mechanism.

摘要

5'-[γ(S)-16O, 17O, 18O]三磷酸腺苷已被合成，并用于确定酵母己糖激酶催化的磷酰基转移的立体化学过程。在环化和甲基化后，通过31P核磁共振光谱分析了形成的D-葡萄糖6-[16O,17O,18O]磷酸酯磷原子处的手性。发现磷酰基转移以构型翻转的方式发生，立体选择性超过94%。对该结果最简单的解释是，磷酰基通过“直线”机制在酶-底物三元复合物中的底物之间转移。

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本文引用的文献

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Crystallographic studies and model building of ATP at the active site of hexokinase.己糖激酶活性位点处ATP的晶体学研究与模型构建。
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Biochemistry. 1980 Jul 22;19(15):3531-6. doi: 10.1021/bi00556a018.
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