Shupnik M A, Antoniades H N, Tashjian A H
Life Sci. 1982 Jan 25;30(4):347-53. doi: 10.1016/0024-3205(82)90571-9.
Human platelet-derived growth factor (PDGF) stimulated the production of prostaglandin E2 (PGE2) by G-292 cells, a clonal line of human osteosarcoma cells. Half-maximal stimulation occurred with 9 ng/ml PDGF and maximal stimulation, 3-fold above control values, occurred with 40 ng/ml of the protein. Treatment of G-292 cells with 40 ng/ml PDGF also reduced the binding of iodinated epidermal growth factor (EGF) to the EGF receptor on G-292 cells. The effect was time-dependent, and EGF binding was reduced to 60% of control by 24-48 h. PDGF did not, however, compete directly for binding to the EGF receptor. The effects of PDGF and EGF on increased PGE2 production appeared to be additive at all concentrations tested, indicating that they may act through a common pathway, but not via the same membrane receptors.
人血小板衍生生长因子（PDGF）刺激人骨肉瘤细胞系G - 292细胞产生前列腺素E2（PGE2）。9 ng/ml的PDGF可产生半数最大刺激作用，40 ng/ml的该蛋白可产生最大刺激作用，刺激程度比对照值高3倍。用40 ng/ml的PDGF处理G - 292细胞也会降低碘化表皮生长因子（EGF）与G - 292细胞上EGF受体的结合。这种作用具有时间依赖性，24 - 48小时后EGF结合减少至对照的60%。然而，PDGF并不直接竞争EGF受体的结合。在所有测试浓度下，PDGF和EGF对PGE2产生增加的作用似乎具有加和性，表明它们可能通过共同途径起作用，但并非通过相同的膜受体。
