Oroszlan S, Sarngadharan M G, Copeland T D, Kalyanaraman V S, Gilden R V, Gallo R C
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1291-4. doi: 10.1073/pnas.79.4.1291.
A human type C retrovirus [human T-cell leukemia (lymphoma) virus; HTLV], recently isolated from young adult patients with cutaneous T-cell lymphoma or leukemia, was not detectably related to other known animal retroviruses in molecular hybridization studies, by comparison of reverse transcriptase and the major core protein p24. The p24 core protein was purified to homogeneity. The amino acid composition, the COOH-terminal amino acid, and the NH(2)-terminal amino acid sequence of the first 25 residues of this major internal structural protein were determined. These results were then compared to the known structure of the internal core protein of other retroviruses. The compositional data reveal that HTLV p24 is chemically distinct from p30-p24 of other animal retroviruses, in agreement with the earlier immunological analyses. However, HTLV p24 shares the common NH(2)-terminal proline and COOH-terminal leucine of all mammalian type C viral p30s. In addition, like bovine leukemia virus (BLV), HTLV lacks the common prolylleucylarginine tripeptide and the larger conserved region found near the NH(2) terminus of the other mammalian type C viral p30s. Alignment of the amino acid sequence of HTLV p24 with previously determined sequences of other retrovirus proteins, including BLV p24, reveals statistically significant sequence homology only to BLV. The results reported here demonstrate that HTLV p24 is related to but chemically distinct from the major core protein of other retroviruses. Similarly, previous results showed that there was no immunological crossreactivity of the p24 protein and reverse transcriptase of HTLV with other retroviruses, including BLV, and no nucleic acid sequence homology. However, the present results, combined with the common size of the p24 and reverse transcriptase, suggest that HTLV may be closer to BLV than any other known retrovirus.
一种人类C型逆转录病毒[人类T细胞白血病（淋巴瘤）病毒；HTLV]，最近从患有皮肤T细胞淋巴瘤或白血病的年轻成年患者中分离出来，在分子杂交研究中，通过比较逆转录酶和主要核心蛋白p24，未发现其与其他已知动物逆转录病毒有明显关联。p24核心蛋白被纯化至同质。确定了该主要内部结构蛋白的氨基酸组成、COOH末端氨基酸以及前25个残基的NH(2)末端氨基酸序列。然后将这些结果与其他逆转录病毒内部核心蛋白的已知结构进行比较。组成数据显示，HTLV p24在化学性质上与其他动物逆转录病毒的p30 - p24不同，这与早期的免疫学分析一致。然而，HTLV p24与所有哺乳动物C型病毒p30s共享共同的NH(2)末端脯氨酸和COOH末端亮氨酸。此外，与牛白血病病毒（BLV）一样，HTLV缺乏其他哺乳动物C型病毒p30s在NH(2)末端附近发现的常见脯氨酰亮氨酰精氨酸三肽和更大的保守区域。将HTLV p24的氨基酸序列与先前确定的其他逆转录病毒蛋白序列（包括BLV p24）进行比对，发现仅与BLV有统计学上显著的序列同源性。此处报道的结果表明，HTLV p24与其他逆转录病毒的主要核心蛋白相关，但在化学性质上不同。同样，先前的结果表明，HTLV的p24蛋白和逆转录酶与包括BLV在内的其他逆转录病毒没有免疫交叉反应，也没有核酸序列同源性。然而，目前的结果与p24和逆转录酶的共同大小相结合，表明HTLV可能比任何其他已知逆转录病毒更接近BLV。
