Kalyanaraman V S, Sarngadharan M G, Nakao Y, Ito Y, Aoki T, Gallo R C
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1653-7. doi: 10.1073/pnas.79.5.1653.
In Japan, adult T-cell leukemias and lymphomas are more common than in the United States and Europe, and in the southwest part of Japan these T-cell malignancy cases appear in clusters. Therefore, we investigated the involvement in these leukemias and lymphomas of the human T-cell leukemia virus (HTLV) that was previously isolated in one of our laboratories from cultured T cells of some patients in the United States with leukemias and lymphomas involving relatively mature T cells. High titers of antibodies capable of quantitative precipitation of (125)I-labeled p24, a well characterized core protein of HTLV, were detected in 12 of 12 patients with untreated adult T-cell leukemia (ATL). (One negative was a patient on chemotherapy.) Ten of the 12 positive samples were from an area where the disease is endemic. Strong precipitating antibodies were also detected in five of seven cases of T-cell malignant lymphoma (TML) which differs from ATL by having fewer leukemic cells in the peripheral blood. High antibody titers were also observed in one of five cases of acute monoblastic leukemia and one of eight cases of chronic myelogenous leukemia in the blast phase of the disease. Low to moderate titers of antibodies were detected in several categories of leukemia (two cases of blast-phase chronic myelogenous leukemia, two cases of acute lymphoblastic leukemia of the null-cell type, and one case of acute myelogenous leukemia). Among all categories of leukemias, except ATL and TML, more cases were negative than positive for anti-p24 activity. All of 79 sera from normal Japanese, including 39 collected from the endemic ATL area of southwest Japan, were negative for antibodies to HTLV p24. All the positive reactivities observed were highly specific to HTLV. The only competition observed in the precipitation of HTLV p24 was with HTLV or with cell lines expressing HTLV and not with various animal retroviruses or a large number of human and subhuman primate cell lines, not known to be producing HTLV. The data strongly indicate an association of HTLV with the increased incidence of ATL in parts of Japan, probably with other forms of leukemias in Japan, and, less commonly, with certain T-cell malignancies in the United States.
在日本，成人T细胞白血病和淋巴瘤比在美国和欧洲更为常见，并且在日本西南部这些T细胞恶性肿瘤病例呈聚集性出现。因此，我们对人类T细胞白血病病毒（HTLV）与这些白血病和淋巴瘤的关联进行了调查，该病毒先前是在我们实验室之一从美国一些患有涉及相对成熟T细胞的白血病和淋巴瘤患者的培养T细胞中分离出来的。在12例未经治疗的成人T细胞白血病（ATL）患者中，有12例检测到能够定量沉淀（125）I标记的p24（HTLV一种特征明确的核心蛋白）的高滴度抗体。（1例阴性患者正在接受化疗。）12份阳性样本中有10份来自该病的地方性流行区域。在7例T细胞恶性淋巴瘤（TML）中，有5例也检测到强沉淀抗体，TML与ATL的不同之处在于外周血中的白血病细胞较少。在5例急性单核细胞白血病患者中的1例以及8例处于疾病急变期的慢性粒细胞白血病患者中的1例也观察到高抗体滴度。在几类白血病（2例急变期慢性粒细胞白血病、2例无核细胞型急性淋巴细胞白血病和1例急性髓细胞白血病）中检测到低至中等滴度的抗体。在所有类型的白血病中，除了ATL和TML，抗p24活性阴性的病例比阳性的更多。来自正常日本人的79份血清，包括从日本西南部ATL地方性流行区域采集的39份血清，对HTLV p24抗体均呈阴性。观察到的所有阳性反应对HTLV都具有高度特异性。在HTLV p24沉淀中观察到的唯一竞争是与HTLV或表达HTLV的细胞系之间的竞争，而不是与各种动物逆转录病毒或大量已知不产生HTLV的人类及类人猿细胞系之间的竞争。数据强烈表明HTLV与日本部分地区ATL发病率的增加有关，可能与日本的其他白血病形式有关，在美国则较少与某些T细胞恶性肿瘤有关。
