Cornwell H J, Koptopoulos G, Thompson H, McCandlish I A, Wright N G
Vet Rec. 1982 Jan 9;110(2):27-32. doi: 10.1136/vr.110.2.27.
Four litters of puppies were divided into three groups. One group was vaccinated with a live CAV-1 vaccine and another with a live CAV-2 vaccine. Throat swabs were collected from two dogs in each of these groups to monitor the possible excretion of vaccine virus, but none was found. Both groups, together with the third group of unvaccinated controls, were challenged 17 days later with an aerosol of virulent CAV-2. One dog from each group was killed on the third, fourth, seventh, ninth, 11th and 14th days after challenge. The unvaccinated dogs developed a clinical disease characterised by anorexia, dullness, coughing and tachypnoea. The lungs were consolidated and histological examination revealed the main lesion to be a severe necrotising bronchiolitis. Large amounts of virus were present in the respiratory tissues of these dogs and high titres of virus were isolated from throat swabs. In contrast, both groups of vaccinated dogs remained clinically almost normal with minimal lesions, present for a much shorter period of time. Virus was found on day 4 in the respiratory tissues of one dog vaccinated with CAV-1 but the other vaccinated animals contained little or no virus. In general, the degree of protection afforded by CAV-1 vaccine seemed similar to that provided by CAV-2 vaccine.
四窝幼犬被分成三组。一组用犬1型腺病毒活疫苗接种，另一组用犬2型腺病毒活疫苗接种。从每组中的两只狗采集咽喉拭子，以监测疫苗病毒的可能排泄情况，但未发现病毒。这两组以及第三组未接种疫苗的对照犬，在17天后用强毒犬2型腺病毒气雾剂进行攻毒。攻毒后第3、4、7、9、11和14天，每组处死一只狗。未接种疫苗的狗出现了以厌食、精神沉郁、咳嗽和呼吸急促为特征的临床疾病。肺发生实变，组织学检查显示主要病变为严重的坏死性细支气管炎。这些狗的呼吸组织中存在大量病毒，咽喉拭子中分离到高滴度病毒。相比之下，两组接种疫苗的狗在临床上几乎保持正常，病变轻微，且持续时间短得多。在接种犬1型腺病毒疫苗的一只狗的呼吸组织中，在第4天发现了病毒，但其他接种疫苗的动物几乎没有或没有病毒。总体而言，犬1型腺病毒疫苗提供的保护程度似乎与犬2型腺病毒疫苗相似。
