Diehl V, Pfreundschuh M, Fonatsch C, Stein H, Falk M, Burrichter H, Schaadt M
Cancer Surv. 1985;4(2):399-419.
Five Hodgkin's disease (HD) derived cell lines were established in vitro in our laboratory in the last seven years. Morphological, cytochemical, immunological and cytogenetic marker analysis demonstrated that the in vitro cells represent genotypically and phenotypically the in vivo Hodgkin (H) and Sternberg-Reed (SR) cells in biopsy specimens. The cultured cells resemble haematolymphoid cells at different stages of maturation. Four of the five continue to grow in vitro as suspension cells after more than 50 months. Four more in vitro HD-derived lines were described recently by several authors. A summary of the various marker characteristics of these in vitro lines is given as a synopsis of the phenotypic marker spectrum and is discussed in comparison with our own cell lines. There is a striking similarity between two of the newly established lines (CO, HDLM-2) and our lines whereas the two other in vitro established cultures seem to resemble cell species further along the line of maturation to B lymphocytes (DEV) and monocytes (SU-HD-1). Gene rearrangement experiments undertaken with the L428, L540, L591 and the CO cell line show that the L428 and 591 cells have undergone gene rearrangement, the L428 being compatible with the genotypic state of a pre-B cell; the L591 cells, similarly rearranged furthermore demonstrated functional light chain rearrangement, compatible with B-cell development. By cytogenetic analysis chromosome 7 was found to be affected in all our described lines. This chromosome appears to be particularly unstable and vulnerable in patients with HD, since all tested cell lines revealed multiple abnormalities of this chromosome, a finding which is in accordance with observations made by other investigators in HD-biopsy cells. Since similar structural changes or loss of chromosome 7 is a characteristic event in cases of secondary acute non-lymphocytic leukaemia, it is speculated that this form of secondary neoplasia could resemble the blast crisis, as observed in chronic myeloid leukaemia.
在过去七年里，我们实验室在体外建立了五株源自霍奇金病（HD）的细胞系。形态学、细胞化学、免疫学及细胞遗传学标记分析表明，体外培养的细胞在基因型和表型上均代表活检标本中的体内霍奇金（H）细胞和施特恩贝格-里德（SR）细胞。培养的细胞类似于处于不同成熟阶段的血液淋巴细胞。五株细胞系中有四株在超过50个月后仍作为悬浮细胞在体外继续生长。最近，几位作者又描述了另外四株源自HD的体外细胞系。这些体外细胞系的各种标记特征总结如下，作为表型标记谱的概要，并与我们自己的细胞系进行比较讨论。新建立的两株细胞系（CO、HDLM-2）与我们的细胞系有显著相似性，而另外两株体外建立的培养物似乎类似于在向B淋巴细胞（DEV）和单核细胞（SU-HD-1）成熟过程中更靠后的细胞类型。对L428、L540、L591和CO细胞系进行的基因重排实验表明，L428和591细胞发生了基因重排，L428与前B细胞的基因型状态相符；L591细胞同样发生了重排，进一步证明了功能性轻链重排，与B细胞发育相符。通过细胞遗传学分析发现，我们描述的所有细胞系中7号染色体均受到影响。在HD患者中，这条染色体似乎特别不稳定且易受损，因为所有检测的细胞系均显示该染色体存在多种异常，这一发现与其他研究者在HD活检细胞中的观察结果一致。由于7号染色体的类似结构变化或缺失是继发性急性非淋巴细胞白血病病例中的特征性事件，因此推测这种继发性肿瘤形式可能类似于慢性髓性白血病中观察到的急变期。
