Roberts A N, Smith K L, Dowell B L, Hubbard A K
Cancer Res. 1978 Sep;38(9):3033-43.
A neoplastic cell line (designated HuT11) has been established in continuous culture from an involved lymph node of a patient with Stage IIA Hodgkin's disease of the mixed cellularity type. The HuT11 line has been morphologically heterogeneous, consisting of mononucleate lymphoid-like cells, polygonal epithelioid cells, and mono-, bi-, and multinucleate giant cells. Four clones initiated from isolated binucleate giant cells of the HuT11 line also have been successfully established as continuous cell lines. The cloned lines have been morphologically distinct and more homogeneous, although typical giant cells have consistently appeared throughout the long-term culture of each. The HuT11 lines have grown as monolayers in McCoy's Medium 5A supplemented with 10% fetal calf serum, with generation times of 12 to 14 hr and high saturation densities. Cytogenetic studies showed that early and later passages of HuT11 cells were aneuploid, and all cell lines were successfully heterotransplanted in the hamster cheek pouch. Repeated indirect immunofluorescence examinations have shown each cell line to be negative for Epstein-Barr virus nuclear antigen. Indirect immunofluorescence tests in which monospecific immunoglobulins were used revealed positive membrane reactions for the gamma (heavy)-chain and kappa (light)-chain of human immunoglobulin G in approximately 20% of viable cells in each line; however, direct immunofluorescence with anti-human immunoglobulin G F(ab')2 reagent failed to confirm these reactions. Rosette tests for B- and T-lymphocyte and macrophage membrane receptors yielded negative results. All cell lines were strongly phagocytic for latex particles and neutral red dye. Cytochemical stains of the monolayers revealed abundant esterase, fluoride-resistant nonspecific esterase, acid phosphatase, and leucine aminopeptidase activities, while lysozyme assays were negative. Although some properties of the HuT11 lines have suggested a macrophage derivation, an undifferentiated lymphoid cell origin of the Hodgkin's neoplastic cell remains a possibility.
已从一名患有 IIA 期混合型霍奇金病患者的受累淋巴结中建立了一种肿瘤细胞系（命名为 HuT11）并进行连续培养。HuT11 细胞系在形态上具有异质性，由单核淋巴样细胞、多角形上皮样细胞以及单核、双核和多核巨细胞组成。从 HuT11 细胞系分离的双核巨细胞起始的四个克隆也已成功建立为连续细胞系。这些克隆系在形态上各不相同且更加均一，尽管在每个克隆系的长期培养过程中始终会出现典型的巨细胞。HuT11 细胞系在添加 10% 胎牛血清的 McCoy's 5A 培养基中呈单层生长，代时为 12 至 14 小时，饱和密度较高。细胞遗传学研究表明，HuT11 细胞的早期和后期传代均为非整倍体，并且所有细胞系都成功地异种移植到了仓鼠颊囊中。反复的间接免疫荧光检查显示每个细胞系的爱泼斯坦 - 巴尔病毒核抗原均为阴性。使用单特异性免疫球蛋白进行的间接免疫荧光试验显示，每个细胞系中约 20% 的活细胞对人免疫球蛋白 G 的γ（重）链和κ（轻）链呈阳性膜反应；然而，用抗人免疫球蛋白 G F(ab')2 试剂进行的直接免疫荧光未能证实这些反应。对 B 淋巴细胞、T 淋巴细胞和巨噬细胞膜受体进行的玫瑰花结试验结果均为阴性。所有细胞系对乳胶颗粒和中性红染料均具有强烈的吞噬作用。单层细胞的细胞化学染色显示有丰富的酯酶、耐氟非特异性酯酶、酸性磷酸酶和亮氨酸氨肽酶活性，而溶菌酶测定为阴性。尽管 HuT11 细胞系的一些特性提示其来源于巨噬细胞，但霍奇金肿瘤细胞起源于未分化淋巴样细胞仍有可能。
