分离的豚鼠肾上腺细胞对促肾上腺皮质激素和阿片促黑激素皮质素原衍生肽的反应。

Responses of isolated guinea-pig adrenal cells to ACTH and pro-opiocortin-derived peptides.

作者信息

Pham-Huu-Trung M T, De Smitter N, Bogio A, Bertagna X, Girard F

出版信息

Endocrinology. 1982 May;110(5):1819-21. doi: 10.1210/endo-110-5-1819.

Abstract

A comparison of the responses of isolated guinea-pig adrenal cells to ACTH and pro-opiocortin-derived peptides was carried out by measuring cortisol, aldosterone, androstenedione and dehydroepiandrosterone production. With concentrations below 10,000 pg/ml, no steroidogenic activity was found in response to either beta-LPH, gamma-LPH, gamma 3-MSH or the 16K fragment, whether assayed alone or in association with ACTH. At concentrations above 10,000 pg/ml, gamma-LPH (100 ng), the 16K fragment (100 ng) and beta-endorphin (500 ng) proved to be totally inactive. beta-LPH from 25 to 250 ng, however, exhibited a significant though slight stimulatory effect on cortisol, aldosterone and androstenedione production. Its effectiveness on aldosterone production was especially marked, but the extent of the response was modest in view of the concentrations used.

摘要

通过测量皮质醇、醛固酮、雄烯二酮和脱氢表雄酮的生成量，对分离的豚鼠肾上腺细胞对促肾上腺皮质激素（ACTH）和阿片促黑皮质素原衍生肽的反应进行了比较。当浓度低于10,000 pg/ml时，无论是单独检测还是与ACTH联合检测，β-促脂素（β-LPH）、γ-促脂素（γ-LPH）、γ3-促黑素（γ3-MSH）或16K片段均未引发类固醇生成活性。当浓度高于10,000 pg/ml时，γ-LPH（100 ng）、16K片段（100 ng）和β-内啡肽（500 ng）被证明完全无活性。然而，25至250 ng的β-LPH对皮质醇、醛固酮和雄烯二酮的生成表现出显著但轻微的刺激作用。其对醛固酮生成的有效性尤为明显，但鉴于所用浓度，反应程度适中。