Shimomura Y, Lee M, Oku J, Bray G A, Glick Z
Metabolism. 1982 Mar;31(3):213-6. doi: 10.1016/0026-0495(82)90055-5.
Groups of hypophysectomized rats were treated with pharmacologic doses of growth hormone, triiodothyronine or cortisone acetate alone or with a combination of growth hormone plus triiodothyronine or growth hormone plus cortisone. After a 7 day period of treatment the hydrolysis of ATP in the presence of ouabain (mg ATPase) and in the absence of ouabain (total ATPase) was determined. Ouabain-suppressible sodium, potassium-dependent ATPase or (Na+ + K+) ATPase was calculated as the difference in the rate of hydrolysis in the presence and absence of ouabain. The activity of the Mg ATPase was significantly reduced in brain after treatment with growth hormone regardless of whether other hormone. In liver there was a significant increase in (Na+ + K+) ATPase in growth hormone, triiodothyronine, or (Na+ + K+) ATPase but there was no effect of triiodothyronine or cortisone and no interaction with the effect of growth hormone. In liver there was a significant increase in (Na+ + K+) ATPase in growth hormone, triiodothyronine, or cortisone-treated animals but Mg ATPase was unaffected by hormone treatment except for the group receiving both growth hormone and cortisone. In kidney homogenates both growth hormone and triiodothyronine significantly increased the activity of the (Na+ + K+) ATPase. There was no effect of cortisone. These data suggest that growth hormone and triiodothyronine may both be calorigenic through their effect on the sodium pumping mechanism in the call membrane.
将垂体切除的大鼠分组，分别单独用药理剂量的生长激素、三碘甲状腺原氨酸或醋酸可的松治疗，或用生长激素加三碘甲状腺原氨酸或生长激素加可的松的组合治疗。治疗7天后，测定在哇巴因存在下（毫克ATP酶）和不存在哇巴因时（总ATP酶）ATP的水解情况。哇巴因抑制的钠钾依赖性ATP酶或（Na + + K +）ATP酶通过存在和不存在哇巴因时水解速率的差异来计算。无论是否使用其他激素，用生长激素治疗后，脑内Mg ATP酶的活性均显著降低。在肝脏中，生长激素、三碘甲状腺原氨酸或（Na + + K +）ATP酶治疗的动物中（Na + + K +）ATP酶显著增加，但三碘甲状腺原氨酸或可的松无此作用，且与生长激素的作用无相互作用。在肝脏中，生长激素、三碘甲状腺原氨酸或可的松治疗的动物中（Na + + K +）ATP酶显著增加，但除了同时接受生长激素和可的松的组外，Mg ATP酶不受激素治疗的影响。在肾匀浆中，生长激素和三碘甲状腺原氨酸均显著增加（Na + + K +）ATP酶的活性。可的松无此作用。这些数据表明，生长激素和三碘甲状腺原氨酸可能都通过对细胞膜钠泵机制的作用而具有产热作用。
