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博德特氏菌属中的相移标记:IV期变体中细胞色素d-629的缺失。

Phase-shift markers in the genus Bordetella: loss of cytochrome d-629 in phase IV variants.

作者信息

Ezzell J W, Dobrogosz W J, Kloos W E, Manclark C R

出版信息

Microbios. 1981;31(125-126):171-81.

PMID:6281628
Abstract

The respiratory electron transport chain of Bordetella pertussis was examined in whole cell suspensions using difference spectra obtained at room temperature. Phase I (virulent) strains were found to possess cytochromes a-603, b-560, c-550, d-629 and cytochrome o. Cytochrome c-553, previously reported (Sutherland, 1963) was not detected and was assumed to be masked by the alpha-peaks for c-550 and b-560. Phase IV (avirulent) strains and C-mode cells (phase I strains grown in the presence of 20 mM MgSO4) were deficient in cytochrome d-629 and appeared to have higher levels of cytochromes a-603, b-560, c-550 and cytochrome o. Preliminary data indicate that B. parapertussis and B. bronchiseptica have electron transport chains similar to that of B. pertussis.

摘要

使用在室温下获得的差示光谱,在全细胞悬液中检测了百日咳博德特氏菌的呼吸电子传递链。发现I相(有毒力)菌株含有细胞色素a-603、b-560、c-550、d-629和细胞色素o。先前报道的(萨瑟兰,1963年)细胞色素c-553未被检测到,推测它被c-550和b-560的α峰掩盖。IV相(无毒力)菌株和C型细胞(在20 mM MgSO4存在下生长的I相菌株)缺乏细胞色素d-629,且似乎具有较高水平的细胞色素a-603、b-560、c-550和细胞色素o。初步数据表明,副百日咳博德特氏菌和支气管败血博德特氏菌具有与百日咳博德特氏菌相似的电子传递链。

相似文献

1
Phase-shift markers in the genus Bordetella: loss of cytochrome d-629 in phase IV variants.博德特氏菌属中的相移标记:IV期变体中细胞色素d-629的缺失。
Microbios. 1981;31(125-126):171-81.
2
Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica.百日咳博德特氏菌、副百日咳博德特氏菌和支气管败血博德特氏菌基因组序列的比较分析。
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Transfer of a pertussis toxin expression locus to isogenic bvg-positive and bvg-negative strains of Bordetella bronchiseptica using an in vivo technique.使用体内技术将百日咳毒素表达基因座转移至支气管败血波氏杆菌的同基因bvg阳性和bvg阴性菌株。
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[Electron transport systems of Photobacterium fischeri].
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引用本文的文献

1
Characterization of the PAS domain in the sensor-kinase BvgS: mechanical role in signal transmission.传感器激酶 BvgS 中 PAS 结构域的特性:在信号转导中的机械作用。
BMC Microbiol. 2013 Jul 24;13:172. doi: 10.1186/1471-2180-13-172.
2
Molecular pathogenesis, epidemiology, and clinical manifestations of respiratory infections due to Bordetella pertussis and other Bordetella subspecies.百日咳博德特氏菌及其他博德特氏菌亚种引起的呼吸道感染的分子发病机制、流行病学及临床表现
Clin Microbiol Rev. 2005 Apr;18(2):326-82. doi: 10.1128/CMR.18.2.326-382.2005.
3
New virulence-activated and virulence-repressed genes identified by systematic gene inactivation and generation of transcriptional fusions in Bordetella pertussis.
通过对百日咳博德特氏菌进行系统的基因失活和转录融合构建而鉴定出的新的毒力激活基因和毒力抑制基因。
J Bacteriol. 2000 Oct;182(20):5902-5. doi: 10.1128/JB.182.20.5902-5905.2000.
4
Genetic analysis of phase change in Bordetella pertussis.百日咳博德特氏菌相变的遗传分析。
Infect Immun. 1984 Jan;43(1):263-9. doi: 10.1128/iai.43.1.263-269.1984.
5
Clonal diversity and host distribution in Bordetella bronchiseptica.支气管败血波氏杆菌的克隆多样性与宿主分布
J Bacteriol. 1987 Jun;169(6):2793-803. doi: 10.1128/jb.169.6.2793-2803.1987.
6
Surface proteins of Bordetella pertussis: comparison of virulent and avirulent strains and effects of phenotypic modulation.百日咳博德特氏菌的表面蛋白:有毒力菌株与无毒力菌株的比较及表型调节的影响
Infect Immun. 1986 Nov;54(2):308-14. doi: 10.1128/iai.54.2.308-314.1986.