胡萝卜软腐欧文氏菌的染色体复制起点（oriC）
The chromosomal origin of replication (oriC) of Erwinia carotovora.
作者信息
Takeda Y, Harding N E, Smith D W, Zyskind J W
出版信息
Nucleic Acids Res. 1982 Apr 24;10(8):2639-50. doi: 10.1093/nar/10.8.2639.
Abstract
The chromosomal DNA replication origin (oriC) of the plant pathogen Erwinia carotovora has been isolated and sequenced. The minimal E. carotovora oriC regional functional in Escherichia coli is a 374 base pair region located on a 7.9 kilobase pair SalI fragment which also contains a functional asnA gene. Differences between the nucleotide sequence of the minimal origin regions of E. carotovora and those of E. coli and Salmonella typhimurium are clustered nucleotide substitutions, with regions of complete homology, up to 19 base pairs long, between the three origins. Nine GATC sites are found in the minimal origin, and all are conserved. In contrast, the region toward asnA from the minimal origin shows little clustering and the differences occur mainly every third nucleotide, suggesting that this region is a protein coding region.
摘要
植物病原菌胡萝卜软腐欧文氏菌的染色体DNA复制起点（oriC）已被分离并测序。在大肠杆菌中具有功能的最小胡萝卜软腐欧文氏菌oriC区域是一个位于7.9千碱基对SalI片段上的374碱基对区域，该片段还包含一个功能性的天冬酰胺合成酶A（asnA）基因。胡萝卜软腐欧文氏菌最小起始区域的核苷酸序列与大肠杆菌和鼠伤寒沙门氏菌的最小起始区域核苷酸序列之间的差异是成簇的核苷酸取代，这三个起始区域之间存在长达19个碱基对的完全同源区域。在最小起始区域发现了9个GATC位点，并且所有位点都是保守的。相比之下，从最小起始区域到asnA的区域几乎没有成簇现象，差异主要每隔第三个核苷酸出现，这表明该区域是一个蛋白质编码区。
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