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微型染色体复制的功能分析:来自大肠杆菌复制起点oriC的双向和单向复制

Functional analysis of minichromosome replication: bidirectional and unidirectional replication from the Escherichia coli replication origin, oriC.

作者信息

Meijer M, Messer W

出版信息

J Bacteriol. 1980 Aug;143(2):1049-53. doi: 10.1128/jb.143.2.1049-1053.1980.

Abstract

Replicating molecules of minichromosomes pCM959 and pOC24 were analyzed by electron microscopy. Replication of pCM959 proceeded bidirectionally from the replication origin, oriC, in about 60% of the molecules; the rest of the molecules replicated unidirectionally in either direction. pOC24, in which deoxyribonucleic acid to the right (clockwise) of the oriC segment is deleted, seemed to replicate predominantly unidirectionally counterclockwise from oriC.

摘要

通过电子显微镜对微型染色体pCM959和pOC24的复制分子进行了分析。在大约60%的分子中,pCM959从复制起点oriC开始双向复制;其余分子则沿任一方向单向复制。pOC24中oriC片段右侧(顺时针方向)的脱氧核糖核酸被删除,它似乎主要从oriC开始逆时针单向复制。

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