Reverse and normal phase h.p.l.c. systems have been developed for separation ranitidine and three metabolites, desmethylranitidine, ranitidine-S-oxide and ranitidine-N-oxide. 2. These h.p.l.c. systems have been evaluated for characterization of ranitidine and metabolites using an h.p.l.c. coupled to a mass spectrometer with a moving belt interface. 3. Ranitidine and its metabolites were thermally degraded under the conditions required to evaporate the reverse phase eluent that contained 40% aq. 0.05 M ammonium acetate. 4. The normal phase eluent was evaporated in the interface at a lower temp. and satisfactory mass spectra were obtained from 1 microgram of ranitidine and each metabolite injected on to the h.p.l.c. column. 5. Normal phase h.p.l.c.-mass spectrometry has been used to identify ranitidine and three of its metabolites in rabbit and human urine obtained after oral administration of ranitidine.
摘要
已开发出反相和正相高效液相色谱系统，用于分离雷尼替丁及其三种代谢物，即去甲雷尼替丁、雷尼替丁 - S - 氧化物和雷尼替丁 - N - 氧化物。2. 这些高效液相色谱系统已通过与带有移动带接口的质谱仪联用的高效液相色谱法，对雷尼替丁及其代谢物进行了表征评估。3. 在蒸发含40%水的0.05M醋酸铵的反相洗脱液所需的条件下，雷尼替丁及其代谢物会发生热降解。4. 正相洗脱液在接口处较低温度下蒸发，从注入高效液相色谱柱的1微克雷尼替丁和每种代谢物中获得了令人满意的质谱图。5. 正相高效液相色谱 - 质谱联用已用于鉴定口服雷尼替丁后从兔和人尿液中提取的雷尼替丁及其三种代谢物。
