Martin L E, Oxford J, Tanner R J
J Chromatogr. 1982 Apr 9;251(2):215-24. doi: 10.1016/s0021-9673(00)98519-4.
High-performance liquid chromatography has been used for the qualitative analysis of metabolites and the quantitative analysis of ranitidine in the urine from subjects given oral and intravenous doses of ranitidine. Ranitidine, ranitidine-N-oxide, ranitidine-S-oxide and desmethylranitidine were identified in extracts of the urine obtained by the XAD-methanol procedure. A selected-ion monitoring technique, using [2H3]ranitidine as the internal standard, was used to determine ranitidine. A normal-phase system consisting of methanol-propan-2-ol-5 M ammonium acetate (50:50:1) was used, and because of this the volume of urine which could be injected on-column without deterioration of the chromatography was limited to 10 microliters. This limited the sensitivity of the method to 1.0 microgram of ranitidine per millilitre of urine.
高效液相色谱法已用于口服和静脉注射雷尼替丁受试者尿液中代谢物的定性分析以及雷尼替丁的定量分析。通过XAD-甲醇法获得的尿液提取物中鉴定出了雷尼替丁、雷尼替丁-N-氧化物、雷尼替丁-S-氧化物和去甲基雷尼替丁。采用以[2H3]雷尼替丁为内标的选择离子监测技术来测定雷尼替丁。使用由甲醇-丙-2-醇-5M醋酸铵（50:50:1）组成的正相系统，因此，在不使色谱恶化的情况下可注入柱中的尿液体积限制为10微升。这将该方法的灵敏度限制为每毫升尿液中1.0微克雷尼替丁。
