通过质谱法测定蛋白质衍生肽和肽混合物的序列。
Determination of the sequences of protein-derived peptides and peptide mixtures by mass spectrometry.
作者信息
Morris H R, Williams D H, Ambler R P
出版信息
Biochem J. 1971 Nov;125(1):189-201. doi: 10.1042/bj1250189.
Abstract
Micro-quantities of protein-derived peptides have been converted into N-acetylated permethyl derivatives, and their sequences determined by low-resolution mass spectrometry without prior knowledge of their amino acid compositions or lengths. A new strategy is suggested for the mass spectrometric sequencing of oligopeptides or proteins, involving gel filtration of protein hydrolysates and subsequent sequence analysis of peptide mixtures. Finally, results are given that demonstrate for the first time the use of mass spectrometry for the analysis of a protein-derived peptide mixture, again without prior knowledge of the protein or components within the mixture.
摘要
微量的蛋白质衍生肽已被转化为N-乙酰化全甲基化衍生物，并通过低分辨率质谱法确定其序列，而无需事先了解其氨基酸组成或长度。本文提出了一种用于寡肽或蛋白质质谱测序的新策略，包括对蛋白质水解产物进行凝胶过滤以及随后对肽混合物进行序列分析。最后，给出的结果首次证明了质谱法可用于分析蛋白质衍生的肽混合物，同样无需事先了解混合物中的蛋白质或成分。
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