Williams J A, Bridge G, Fowler L J, John R A
Biochem J. 1982 Jan 1;201(1):221-5. doi: 10.1042/bj2010221.
Rat liver ornithine aminotransferase is found to exchange the pro-S hydrogen on the delta-carbon atom of ornithine exclusively, thus showing that the mammalian enzyme transfers the delta-amino group and not the alpha-amino group as has been demonstrated with the plant enzyme [Mestichelli, Gupta & Spenser (1979) J. Biol. Chem. 254, 640-647]. The enzyme also transfers the alpha-amino group of glutamate and the kinetics of the half reactions between the enzyme and both amino acids are compared. Rate and dissociation constants for both reactions are determined.
已发现大鼠肝脏鸟氨酸转氨酶仅交换鸟氨酸δ碳原子上的前-S氢,从而表明该哺乳动物酶转移的是δ-氨基而非α-氨基,正如植物酶所证实的那样[梅斯蒂凯利、古普塔和斯彭斯(1979年)《生物化学杂志》254卷,640 - 647页]。该酶还转移谷氨酸的α-氨基,并比较了酶与这两种氨基酸之间半反应的动力学。测定了两个反应的速率和离解常数。