影响大鼠离体肝线粒体中鸟氨酸转氨酶活性的因素
Factors influencing the activity of ornithine aminotransferase in isolated rat liver mitochondria.
作者信息
McGivan J D, Bradford N M, Beavis A D
出版信息
Biochem J. 1977 Jan 15;162(1):147-56. doi: 10.1042/bj1620147.
Abstract
- The characteristics of ornithine catabolism by the aminotransferase pathway in isolated mitochondria were determined. 2. Ornithine synthesis from glutamate and glutamate gamma-semialdehyde produced by the oxidation of proline was studied. No ornithine was formed in the absence of rotenone. 3. The mechanism of ornithine transport was reinvestigated, and the existence of an ornithine+/H+ exchange system postulated. 4. The kinetics of ornithine transport, ornithine catabolism in intact mitochondria and ornithine aminotransferase activity in solubilized mitochondria were compared. It is concluded that ornithine aminotransferase activity in liver mitochondria is rate-limited by the transport of ornithine across the mitochondrial membrane, and that this enzyme is involved primarily in ornithine degradation rather than ornithine synthesis.
摘要
- 测定了分离线粒体中鸟氨酸通过转氨酶途径进行分解代谢的特征。2. 研究了由脯氨酸氧化产生的谷氨酸和谷氨酸γ-半醛合成鸟氨酸的过程。在没有鱼藤酮的情况下未形成鸟氨酸。3. 重新研究了鸟氨酸转运机制，并推测存在鸟氨酸+/H+交换系统。4. 比较了鸟氨酸转运的动力学、完整线粒体中鸟氨酸的分解代谢以及溶解线粒体中鸟氨酸转氨酶的活性。得出的结论是，肝线粒体中的鸟氨酸转氨酶活性受鸟氨酸跨线粒体膜转运的限速，并且该酶主要参与鸟氨酸的降解而非合成。
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