电子顺磁共振光谱法提供的证据表明，亚硫酸根离子与亚硫酸氧化酶的钼中心形成了复合物。
Evidence from electron-paramagnetic-resonance spectroscopy for a complex of sulphite ions with the molybdenum centre of sulphite oxidase.
作者信息
Bray R C, Lamy M T, Gutteridge S, Wilkinson T
出版信息
Biochem J. 1982 Jan 1;201(1):241-3. doi: 10.1042/bj2010241.
Abstract
Reduction of sulphite oxidase by sulphite at low pH values in Mes (4-morpholine-ethanesulphonic acid) buffer gives rise to a new molybdenum(V) electron-paramagnetic-resonance spectrum different from that obtained by photoreduction of the enzyme in the same medium. The spectrum is attributed to a sulphite complex of the enzyme, showing g-values of about 2.000, 1.972 and 1.963. The complex is analogous to that with the inhibitor phosphate in that it gives rise to no observable hyperfine coupling of Mo(V) to exchangeable protons.
摘要
在Mes（4-吗啉乙磺酸）缓冲液中，低pH值下亚硫酸盐对亚硫酸盐氧化酶的还原产生了一种新的钼（V）电子顺磁共振谱，该谱不同于在相同介质中通过光还原酶所获得的谱。该谱归因于酶的亚硫酸盐复合物，其g值约为2.000、1.972和1.963。该复合物与抑制剂磷酸盐形成的复合物类似，因为它不会导致钼（V）与可交换质子产生可观察到的超精细偶合。
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本文引用的文献
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