Mitsialis S A, Young J F, Palese P, Guntaka R V
Gene. 1981 Dec;16(1-3):217-25. doi: 10.1016/0378-1119(81)90078-0.
A sequence in the long terminal repeat (LTR) of avian tumor virus (ATV) DNA was shown to contain a promoter acute in Escherichia coli. For this analysis the bacterial promoters for the tetracycline (Tc) and neomycin (Nm) resistance genes were deleted from different plasmids and replaced with various fragments derived from the ATV DNA. Expression of the drug-resistant phenotype in the recombinant plasmids at levels comparable to or greater than those found with parental bacterial promotes was shown to be dependent on the presence of an intact sequence ranging from nucleotide +19 to -23 (relative to the cap site) in the ATV DNA. Comparison of the consensus bacterial promoter with the nucleotide sequence in this region revealed strong similarities.
禽肿瘤病毒（ATV）DNA的长末端重复序列（LTR）中的一个序列被证明在大肠杆菌中含有一个急性启动子。为了进行该分析，从不同质粒中删除了四环素（Tc）和新霉素（Nm）抗性基因的细菌启动子，并用源自ATV DNA的各种片段进行替换。重组质粒中耐药表型的表达水平与亲本细菌启动子相当或更高，这表明其依赖于ATV DNA中从核苷酸+19至-23（相对于帽位点）的完整序列的存在。将共有细菌启动子与该区域的核苷酸序列进行比较，发现了很强的相似性。
