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猿猴病毒40启动子指导四环素基因在质粒pACYC184中的表达。

Simian virus 40 promoters direct expression of the tetracycline gene in plasmid pACYC184.

作者信息

Jenkins F J, Howett M K, Rapp F

出版信息

J Virol. 1983 Jan;45(1):478-81. doi: 10.1128/JVI.45.1.478-481.1983.

DOI:10.1128/JVI.45.1.478-481.1983
PMID:6296456
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256434/
Abstract

Insertion of HindIII DNA fragments into the HindIII site of plasmid pACYC184 destroys the promoter of the plasmid tetracycline resistance gene and causes Escherichia coli cells harboring recombinant plasmids to be tetracycline sensitive and chloramphenicol resistant. The HindIII-C DNA fragment of simian virus 40 contains the two virus promoters and the virus origin of replication. We report the isolation of recombinant plasmids that contained the simian virus 40 HindIII-C DNA fragment at the HindIII site but were capable of conferring tetracycline resistance to E. coli cells. The viral promoter sequences contained in the HindIII-C fragment presumably replaced the inactivated tetracycline resistance gene promoter sequences and enabled transcription of the tetracycline resistance gene.

摘要

将HindIII DNA片段插入质粒pACYC184的HindIII位点会破坏质粒四环素抗性基因的启动子，并使携带重组质粒的大肠杆菌细胞对四环素敏感但对氯霉素有抗性。猴病毒40的HindIII-C DNA片段包含两个病毒启动子和病毒复制起点。我们报告了在HindIII位点含有猴病毒40 HindIII-C DNA片段但能够赋予大肠杆菌细胞四环素抗性的重组质粒的分离。HindIII-C片段中包含的病毒启动子序列大概取代了失活的四环素抗性基因启动子序列，并使四环素抗性基因得以转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0a/256434/fc684f5d9dc7/jvirol00148-0495-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0a/256434/fc684f5d9dc7/jvirol00148-0495-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0a/256434/fc684f5d9dc7/jvirol00148-0495-a.jpg

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J Virol. 1983 Jan;45(1):478-81. doi: 10.1128/JVI.45.1.478-481.1983.
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本文引用的文献

1
Isolation and Mapping of Small Cauliflower Mosaic Virus DNA Fragments Active as Promoters in Escherichia coli.在大肠杆菌中作为启动子具有活性的小花椰菜花叶病毒DNA小片段的分离与定位
J Virol. 1981 Feb;37(2):673-82. doi: 10.1128/JVI.37.2.673-682.1981.
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Organization of transcriptional signals in plasmids pBR322 and pACYC184.质粒pBR322和pACYC184中转录信号的组织方式。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):167-71. doi: 10.1073/pnas.78.1.167.
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An avian tumor virus promoter directs expression of plasmid genes in Escherichia coli.
一种禽肿瘤病毒启动子指导质粒基因在大肠杆菌中的表达。
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Cloning a promoter that puts the expression of tetracycline resistance under the control of the regulatory elements of the mer operon.克隆一个启动子，该启动子可使四环素抗性的表达受mer操纵子调控元件的控制。
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Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313.鉴定pBR313中四环素抗性基因的野生型和突变型启动子。
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Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4.含有噬菌体T4 rII基因的重组质粒的构建与特性
Mol Gen Genet. 1978 Feb 27;159(3):301-9. doi: 10.1007/BF00268267.
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Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.新型克隆载体的构建与特性分析。I. 质粒pMB9的氨苄青霉素抗性衍生物
Gene. 1977;2(2):75-93. doi: 10.1016/0378-1119(77)90074-9.
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Altered tetracycline resistance in pSC101 recombinant plasmids.pSC101重组质粒中四环素抗性的改变
Mol Gen Genet. 1977 Mar 16;151(3):327-31. doi: 10.1007/BF00268797.
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Cloning of herpes simplex type 1 DNA fragments in a bacteriophage lambda vector.1型单纯疱疹病毒DNA片段在λ噬菌体载体中的克隆
Science. 1979 Feb 9;203(4380):541-4. doi: 10.1126/science.216076.