Granneman G R, Sennello L T
J Chromatogr. 1982 Apr 16;229(1):149-57. doi: 10.1016/s0378-4347(00)86046-6.
A simple and very precise high-performance liquid chromatographic procedure has been developed for the determination of cefmenoxime, a new broad spectrum cephalosporin antibiotic, in plasma. The workup procedure involves ultrafiltration of samples which have been treated with sodium dodecyl sulfate to displace the drug from its binding sites on plasma proteins. The ultrafiltrates are then directly injected into a high-performance liquid chromatographic system utilizing a reversed-phase analytical column, and an ultraviolet spectrophotometric detector. The mean assay coefficient of variation over a concentration range of 0.5-200 micrograms/ml is slightly greater than 1% when either p-nitrobenzoic or p-anisic acid is used as the internal standard. Recoveries of drug are essentially quantitative at all levels investigated; hence the calibration curves are rectilinear from the limit of quantification (about 0.05 microgram/ml) to at least 200 micrograms/ml.
已开发出一种简单且非常精确的高效液相色谱法，用于测定血浆中新型广谱头孢菌素抗生素头孢甲肟。后处理程序包括对用十二烷基硫酸钠处理过的样品进行超滤，以将药物从其在血浆蛋白上的结合位点置换出来。然后将超滤物直接注入使用反相分析柱和紫外分光光度检测器的高效液相色谱系统中。当使用对硝基苯甲酸或对茴香酸作为内标时，在0.5 - 200微克/毫升的浓度范围内，平均测定变异系数略大于1%。在所研究的所有水平上，药物回收率基本为定量；因此，校准曲线从定量限（约0.05微克/毫升）到至少200微克/毫升呈直线关系。
