Granneman G R, Sennello L T
J Pharm Sci. 1982 Oct;71(10):1112-5. doi: 10.1002/jps.2600711008.
A simple and precise high-performance liquid chromatographic (HPLC) procedure was developed for the determination of cefsulodin, a new antipseudomonal cephalosporin antibiotic, in plasma. The analytical procedure involved ultrafiltration of samples that were buffered to prevent cefsulodin degradation, followed by injection into an HPLC system, utilizing a C18 reversed-phase analytical column, a mobile phase of acetonitrile-modified aqueous acetate buffer, and a UV spectrophotometric detector. Because of the simplicity of the procedure, the intraassay (or approximately 2%) and interassay (or approximately 3-4%) coefficients of variation were extremely low. Recoveries of drug were essentially quantitative in freshly buffered specimens and in those stored buffered and frozen for nearly 3 months. Calibration curves were rectilinear from the limit of quantification (or approximately 0.2 microgram/ml) to 200 micrograms/ml, as demonstrated by regression correlation coefficients averaging greater than 0.999 during routine analyses.
已开发出一种简单而精确的高效液相色谱（HPLC）方法，用于测定血浆中一种新型抗假单胞菌头孢菌素抗生素头孢磺啶。该分析方法包括对样品进行超滤，样品经缓冲以防止头孢磺啶降解，然后注入HPLC系统，使用C18反相分析柱、乙腈改性的醋酸盐水溶液缓冲液作为流动相以及紫外分光光度检测器。由于该方法操作简单，批内变异系数（约2%）和批间变异系数（约3 - 4%）极低。在新鲜缓冲标本以及储存于缓冲状态并冷冻近3个月的标本中，药物回收率基本为定量。校准曲线在定量限（约0.2微克/毫升）至200微克/毫升范围内呈直线，在常规分析期间回归相关系数平均大于0.999即证明了这一点。
