Eick D, Doerfler W
J Virol. 1982 Apr;42(1):317-21. doi: 10.1128/JVI.42.1.317-321.1982.
Approximately 20 to 22 copies of adenovirus type 12 (Ad12) DNA per cell were integrated into the genome of the cell line T637. Only a few of these copies seemed to remain intact and colinear with virion DNA. All other persisting viral genomes exhibited deletions or inversions or both in the right-hand part of Ad12 DNA. Spontaneously arising morphological revertants of T637 cells has lost viral DNA. In most of the revertant cell lines only the intact or a part of the intact viral genome was preserved; other revertant cell lines has lost all viral DNA. In three other Ad12-transformed hamster cell lines, HA12/7, A2497-3, and CLAC3 (Stabel et al., J. Virol. 36:22-40, 1980), major rearrangements at the right end of the integrated Ad12 DNA were not found. These studies were performed to investigate the phenomena of amplification, rearrangements, and deletions of Ad12 DNA in hamster cells.
每个细胞中约有20至22份12型腺病毒（Ad12）DNA整合到细胞系T637的基因组中。这些拷贝中只有少数似乎保持完整并与病毒粒子DNA共线。所有其他持续存在的病毒基因组在Ad12 DNA的右侧部分表现出缺失、倒位或两者皆有。T637细胞自发产生的形态学回复突变体已丢失病毒DNA。在大多数回复突变细胞系中，仅保留了完整的或部分完整的病毒基因组；其他回复突变细胞系已丢失了所有病毒DNA。在另外三个Ad12转化的仓鼠细胞系HA12/7、A2497-3和CLAC3（Stabel等人，《病毒学杂志》36:22-40，1980）中，未发现整合的Ad12 DNA右端有主要重排。进行这些研究是为了调查仓鼠细胞中Ad12 DNA的扩增、重排和缺失现象。
