Vogel S, Brötz M, Kruczek I, Neumann R, Eick D, Winterhoff U, Doerfler W
Gene. 1981 Nov;15(2-3):273-8. doi: 10.1016/0378-1119(81)90136-0.
The following restriction endonuclease fragments of human adenovirus type 12 (Ad12) DNA have been cloned in plasmid or bacteriophage lambda vectors using standard protocols: the EcoRI-A*, -B, -D, -E, and -F fragments, the BamHI-B, -C, -D, -F, -G, -H, and -I fragments, the HindIII-F and -I fragments, and the PstI-A, -D, -F, -G, and -H fragments. The EcoRI-A* fragment comprises the right terminal 5 kb of Ad12 DNA including the terminal 143 bp.
利用标准方案,已将人12型腺病毒(Ad12)DNA的以下限制性内切酶片段克隆到质粒或噬菌体λ载体中:EcoRI - A*、- B、- D、- E和 - F片段,BamHI - B、- C、- D、- F、- G、- H和 - I片段,HindIII - F和 - I片段,以及PstI - A、- D、- F、- G和 - H片段。EcoRI - A*片段包含Ad12 DNA右侧末端5 kb,包括末端143 bp。