Park M, Kitchener H C, Macnab J C
EMBO J. 1983;2(7):1029-34. doi: 10.1002/j.1460-2075.1983.tb01541.x.
DNA extracted from eight human cervical carcinomas, one lymph node metastasis and related control tissue was examined for the presence of herpes simplex virus (HSV) DNA sequences. Southern blot transfers of tumour and control DNA were hybridised with radioactively labelled cloned probes representing 70% of the HSV-2 genome. Specific hybridisation to HSV DNA sequences was observed in one of eight carcinoma tissues analysed. Hybridisation of HSV-2 DNA probes to BamHI and XhoI restriction enzyme fragments of tumour cell DNA which co-migrated with authentic HSV-2 viral fragments identified co-linear HSV-2 DNA sequences comprising 3% of the HSV-2 genome, between map coordinates 0.582 and 0.612. The remaining eight tumour and all control tissues analysed, showed no specific hybridisation to any of the probes used at levels of sensitivity which would detect 0.5 copies/cell of HSV-2 DNA restriction fragments of 2 kb or greater.
从8例人类宫颈癌、1例淋巴结转移癌及相关对照组织中提取DNA，检测单纯疱疹病毒（HSV）DNA序列的存在情况。用代表HSV - 2基因组70%的放射性标记克隆探针与肿瘤和对照DNA的Southern印迹转移膜进行杂交。在分析的8个癌组织中的1个中观察到与HSV DNA序列的特异性杂交。HSV - 2 DNA探针与肿瘤细胞DNA的BamHI和XhoI限制性酶切片段杂交，这些片段与 authentic HSV - 2病毒片段共迁移，鉴定出共线性HSV - 2 DNA序列，其占HSV - 2基因组的3%，位于图谱坐标0.582和0.612之间。其余8个肿瘤组织和所有分析的对照组织，在能检测到2 kb或更大的HSV - 2 DNA限制性片段0.5拷贝/细胞的灵敏度水平下，未显示与所用任何探针的特异性杂交。
