HeLa细胞7S RNA的cDNA拷贝的克隆与特性分析
Cloning and characterization of cDNA copies of the 7S RNAs of HeLa cells.
作者信息
Ullu E, Melli M
出版信息
Nucleic Acids Res. 1982 Apr 10;10(7):2209-23. doi: 10.1093/nar/10.7.2209.
Abstract
We have cloned cDNA copies of in vitro adenylated 7S RNA of HeLa cells. The most representative clones in the library contain DNA fragments copied from the 7SL and 7SK small RNAs. The two classes of recombinants share no homology. The 7SL RNA contains at the 5' end of the molecule sequences homologous to the Alu sequence family. Hybridization to human genomic DNA shows that the 7SL and 7SK clones are homologous to two different families of repetitive sequences.
摘要
我们已经克隆了HeLa细胞体外腺苷酸化7S RNA的cDNA拷贝。文库中最具代表性的克隆包含从7SL和7SK小RNA复制而来的DNA片段。这两类重组体没有同源性。7SL RNA在分子的5'端含有与Alu序列家族同源的序列。与人类基因组DNA杂交表明，7SL和7SK克隆与两个不同的重复序列家族同源。
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