人类基因组中存在高度重复且广泛分布的DNA序列。
Presence of a highly repetitive and widely dispersed DNA sequence in the human genome.
作者信息
Tashima M, Calabretta B, Torelli G, Scofield M, Maizel A, Saunders G F
出版信息
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1508-12. doi: 10.1073/pnas.78.3.1508.
A genomic DNA library consisting of human DNA fragments about 18 kilobases long cloned in a bacteriophage lambda vector was found to contain a specific repeated DNA segment. The repeated sequence is present in greater than 95% of the genomic library, and selected clones contain at least two copies of the sequence. Our experiments indicate that this highly repetitive sequence (approximately 400,000 copies per haploid genome) is widely distributed in the human genome and is represented in the cytoplasmic polysomal mRNA. This sequence is homologous to the 300-base-pair Alu repeat family, the predominant repeat sequence in man.
一个由约18千碱基长的人类DNA片段克隆于噬菌体λ载体中组成的基因组DNA文库，被发现含有一个特定的重复DNA片段。该重复序列存在于超过95%的基因组文库中，且挑选出的克隆至少含有该序列的两个拷贝。我们的实验表明，这种高度重复序列（单倍体基因组中约有400,000个拷贝）在人类基因组中广泛分布，并存在于细胞质多聚核糖体mRNA中。该序列与300碱基对的Alu重复家族同源，Alu重复家族是人类中主要的重复序列。
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