Gesell M S, Brandes M J, Arnold E A, Isaacs J T, Ueda H, Millan J C, Brandes D
Prostate. 1982;3(2):131-8. doi: 10.1002/pros.2990030205.
Sucrose density gradient analysis of cytosol from normal and neoplastic rat prostatic tissues exhibited a peak of (3H) retinoic acid binding in the 2S region, corresponding to the cytoplasmic retinoic acid binding protein (cRABP). In the Fisher-Copenhagen F1 rat, cRABP was present in the lateral lobe, but could not be detected in the ventral nor in the dorsal prostatic lobes. Four sublines of the R-3327 rat prostatic tumor contained similar levels of this binding protein. The absence of cRABP in the normal tissue of origin of the R-3327 tumor, the rat dorsal prostate, and reappearance in the neoplastic tissues follows a pattern described in other human and animal tumors. The occurrence of cRABP in the well-differentiated as well as in the anaplastic R-3327 tumors in which markers which reflect a state of differentiation and hormonal regulation, such as androgen receptor, 5 alpha reductase, and secretory acid phosphatase are either markedly reduced or absent, points to cRABP as a marker of malignant transformation.
对正常和肿瘤性大鼠前列腺组织的胞质溶胶进行蔗糖密度梯度分析，结果显示在2S区域出现了一个（3H）视黄酸结合峰，这与细胞质视黄酸结合蛋白（cRABP）相对应。在费希尔-哥本哈根F1大鼠中，cRABP存在于侧叶，但在腹侧和背侧前列腺叶中未检测到。R-3327大鼠前列腺肿瘤的四个亚系含有相似水平的这种结合蛋白。R-3327肿瘤的起源正常组织（大鼠背侧前列腺）中不存在cRABP，而在肿瘤组织中重新出现，这与其他人类和动物肿瘤中描述的模式一致。cRABP在分化良好以及间变的R-3327肿瘤中均有出现，在这些肿瘤中，反映分化状态和激素调节的标志物，如雄激素受体、5α还原酶和分泌性酸性磷酸酶，要么明显减少，要么缺失，这表明cRABP是恶性转化的一个标志物。
