Petkov A, Lulov R
Vet Med Nauki. 1981;18(9):37-42.
A fast and readily applicable method is suggested for the determination of the biochemical reactions of the spore producing anaerobic organisms. A pepton coloid medium with the addition of 0.15 per cent agar-agar was used as a basic nutrient medium. The cultivation of bacteria did not require any special conditions. Reading of the results was carried out with the addition of 0.1 per cent phenol red after 48 hours of incubation of the cultures. This method showed a number of advantages over the methods used so far.
本文提出了一种快速且易于应用的方法,用于测定产芽孢厌氧菌的生化反应。以添加了0.15%琼脂的蛋白胨胶体培养基作为基础营养培养基。细菌培养不需要任何特殊条件。在培养物孵育48小时后,加入0.1%的酚红进行结果判读。与目前使用的方法相比,该方法具有许多优点。