Bittner J
Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1975 Jan-Mar;20(1):55-63.
The description is given of a modified method starting from the alkaline pyrogalol technique, described by Béerens and Tahon-Castel, for obtaining anaerobiosis in Petri dishes. The modification consists in: sealing the dishes with paraffin, adding of talcum in the reducing mixture instead of silica, and use of the ready prepared mixture ovar a period of at most 6 months. The method was tested against the reference method using suspensions of C. Oedematiens (species demanding strictly anaerobiosis conditions), C. histolyticum (somewhat less exigent) and C. perfringens spores (mean esigence), seeding on the surface of dishes with Willis-Hobbs medium. The comparative experiment, counting 30,941 colonies on 408 Petri dishes proved the value of the method proposed. The procedure was just as efficient in surface cultures of nonsporulate anaerobic germs.
本文描述了一种从贝伦斯和塔洪 - 卡斯泰尔所描述的碱性连苯三酚技术改进而来的方法，用于在培养皿中实现厌氧环境。改进之处包括：用石蜡密封培养皿，在还原混合物中添加滑石粉而非二氧化硅，以及使用已制备好的混合物，最长使用期限为6个月。使用水肿梭菌（严格要求厌氧条件的菌种）、溶组织梭菌（要求稍低）和产气荚膜梭菌孢子（要求中等）的悬浮液，在威利斯 - 霍布斯培养基表面接种于培养皿上，以此方法与参考方法进行测试对比。在408个培养皿上计数30941个菌落的对比实验证明了所提方法的价值。该程序在非芽孢厌氧菌的表面培养中同样有效。
