Dimitrov P
Vet Med Nauki. 1981;18(9):71-8.
Studies were carried out on the inhibiting activity of amantadine - 1-amino adamantine (1-AA) on the virus of swine transmissive gastroenteritis, Rakovski strain, in cell cultures of pig kidney and thyroid gland. The inhibiting agent was tested in concentration of 6.25 to 100 microgram/cm3. Parallel titrations with and without 1-AA (25, 50, and 100 microgram/cm3) were used to establish the maximum difference in the titers 2 lg. The effect of inhibition was manifested at minimum concentration of 1-AA (12.5 microgram/cm3), and increased up to a concentration of 100 microgram/cm3. Within the interval 12.5-25.00-50.00 microgram/cm3 the doubling of the concentration of the inhibitor led to the decrease in the virus titer by 1 lg. In experiments to follow up the dynamics of the virus growth in the course of four days with and without 1-AA at the rate of 100 microgram/cm3 at varying multiplicity of infecting (from 0.5 up to 0.00005) the transient differences between the titers rose to 4.5 lg, while the differences between the peak values of the titer rose up to 2.5 lg. By means of seven consecutive passage of two variants of the virus selected by their decrease or increase in sensitivity to the inhibiting agent no change in their sensitivity to 1-AA was achieved.
开展了关于金刚烷胺 - 1 - 氨基金刚烷（1 - AA）对猪传染性胃肠炎病毒拉科夫斯基毒株在猪肾和甲状腺细胞培养物中的抑制活性的研究。抑制试剂的测试浓度为6.25至100微克/立方厘米。使用含和不含1 - AA（25、50和100微克/立方厘米）的平行滴定法来确定滴度的最大差异为2对数。抑制作用在1 - AA的最低浓度（12.5微克/立方厘米）时显现，并随着浓度增加至100微克/立方厘米而增强。在12.5 - 25.00 - 50.00微克/立方厘米的浓度区间内，抑制剂浓度加倍会导致病毒滴度降低1对数。在后续实验中，以100微克/立方厘米的浓度，在不同感染复数（从0.5至0.00005）下，跟踪有和没有1 - AA时病毒在四天内的生长动态，滴度之间的瞬时差异升至4.5对数，而滴度峰值之间的差异升至2.5对数。通过对根据对抑制剂敏感性降低或增加而选择的两种病毒变体进行连续七次传代，未实现它们对1 - AA敏感性的改变。
