Nguyen T D, Bottreau E, Aynaud J M
INRA, Laboratoire de Pathologie Porcine, Nouzilly, France.
Ann Rech Vet. 1987;18(3):255-9.
Effect of Concanavalin (ConA) on attachment of three strains of Transmissible Gastroenteritis coronavirus (TGE) of swine was investigated in cell culture. Whatever the virus strain, reduction of virus plaques number is observed when viral suspension is incubated with cells together or after addition of ConA. Intensity of inhibition is related with ConA concentration. ConA treatment of preinfected cell cultures has no effect on plaque formation. Addition of alpha-methyl-D-mannoside inhibits the ConA activity. Treatment of cells with metaperiodate has no effect on plaque formation and ConA activity. Our results suggest that ConA inhibits formation of plaques by TGE coronavirus.
在细胞培养中研究了伴刀豆球蛋白A（ConA）对三株猪传染性胃肠炎冠状病毒（TGE）附着的影响。无论病毒株如何，当病毒悬液与细胞一起孵育或添加ConA后，都会观察到病毒蚀斑数量减少。抑制强度与ConA浓度有关。用ConA处理预先感染的细胞培养物对蚀斑形成没有影响。添加α-甲基-D-甘露糖苷可抑制ConA活性。用高碘酸盐处理细胞对蚀斑形成和ConA活性没有影响。我们的结果表明，ConA抑制TGE冠状病毒蚀斑的形成。
