Cromwell O, Walport M J, Taylor G W, Morris H R, O'Driscoll B R, Kay A B
Adv Prostaglandin Thromboxane Leukot Res. 1982;9:251-7.
Sputum extracts from 16 of 25 adult patients with CF produced a slow, sustained contraction of isolated guinea pig ileum which was partially inhibitable by the SRS-A antagonist FPL 55712. Following progressive purification and HPLC, the major ileum-contracting substance was identified as LTD4. LTC4 was also present but in smaller amounts. The sputum contained other SRS-A-like activity which was not inhibited by FPL 55712; this substance was of a smaller molecular size than LTD4. The presence of LTB4 was also established using HPLC and an assay of neutrophil chemotaxis. These studies raise the possibility that leukotrienes and other lipoxygenase products may contribute to the progressive lung disease characteristics of CF.
25例成年囊性纤维化（CF）患者中，16例患者的痰液提取物可使离体豚鼠回肠产生缓慢、持续的收缩，该收缩可被SRS-A拮抗剂FPL 55712部分抑制。经过逐步纯化和高效液相色谱法（HPLC）分析，主要的回肠收缩物质被鉴定为白三烯D4（LTD4）。白三烯C4（LTC4）也存在，但含量较少。痰液中还含有其他类似SRS-A的活性物质，该物质不被FPL 55712抑制；其分子大小比LTD4小。通过HPLC和中性粒细胞趋化性测定也证实了白三烯B4（LTB4）的存在。这些研究提示白三烯和其他脂氧合酶产物可能与CF进行性肺部疾病特征有关。
