采用非特异性抗血清和部分纯化的活性酶，通过一种新的酶免疫测定方法对聚丙烯酰胺凝胶电泳后的无活性酶蛋白进行特异性检测：应用于大鼠肝脏磷酸二酯酶I - Suppr

Suppr

超能文献

Specific detection of inactive enzyme protein after polyacrylamide gel electrophoresis by a new enzyme-immunoassay method using unspecific antiserum and partially purified active enzyme: application to rat liver phosphodiesterase I.

作者信息

Muilerman H G, ter Hart H G, Van Dijk W

出版信息

Anal Biochem. 1982 Feb;120(1):46-51. doi: 10.1016/0003-2697(82)90315-3.

DOI:10.1016/0003-2697(82)90315-3

PMID:6283933

Abstract

摘要

相似文献

Specific detection of inactive enzyme protein after polyacrylamide gel electrophoresis by a new enzyme-immunoassay method using unspecific antiserum and partially purified active enzyme: application to rat liver phosphodiesterase I.采用非特异性抗血清和部分纯化的活性酶，通过一种新的酶免疫测定方法对聚丙烯酰胺凝胶电泳后的无活性酶蛋白进行特异性检测：应用于大鼠肝脏磷酸二酯酶I

Anal Biochem. 1982 Feb;120(1):46-51. doi: 10.1016/0003-2697(82)90315-3.

On the presence of two non-specific nucleotide-sugar-hydrolysing enzymes in rat liver.大鼠肝脏中两种非特异性核苷酸糖水解酶的存在

Biochem J. 1984 May 15;220(1):95-103. doi: 10.1042/bj2200095.

Cell cycle and 5'-Nucleotide phosphodiesterase activities in rat liver.

Cytometry. 1982 May;2(6):414-20. doi: 10.1002/cyto.990020610.

Modulation of alkaline phosphodiesterase I in cultured rat hepatocytes.

Biochim Biophys Acta. 1987 Apr 22;928(2):144-51. doi: 10.1016/0167-4889(87)90115-7.

Phosphodiesterase-phosphomonoesterases from Fusarium moniliforme. VI. A modified method of purification and identification of isozymes.

J Biochem. 1977 Jun;81(6):1691-7. doi: 10.1093/oxfordjournals.jbchem.a131628.

Two molecular forms of the isolated brain enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase.

FEBS Lett. 1981 Aug 17;131(1):37-40. doi: 10.1016/0014-5793(81)80882-4.

Purification of a phosphodiesterase from Bothrops atrox venom by affinity chromatography.

Eur J Biochem. 1973 Feb 1;32(3):479-85. doi: 10.1111/j.1432-1033.1973.tb02631.x.

The synthesis of 5-iodoindoxyl-3-phenylphosphonate and its use in analysis of phosphodiesterase I.

Anal Biochem. 1990 Feb 15;185(1):90-3. doi: 10.1016/0003-2697(90)90260-g.

Carbamoyl-phosphate synthase (ammonia) of rat and axolotl liver: determination of immunological cross-reactivity without purification of the axolotl enzyme.大鼠和蝾螈肝脏中的氨甲酰磷酸合成酶（氨）：无需纯化蝾螈酶即可测定免疫交叉反应性。

J Exp Zool. 1982 Oct 10;223(2):133-7. doi: 10.1002/jez.1402230205.

Purification and properties of bovine liver lysosomal adenosine 5'-phosphosulphate sulphohydrolase. A non-specific enzyme with pyrophosphatase and phosphodiesterase activities.牛肝溶酶体腺苷5'-磷酸硫酸水解酶的纯化及性质。一种具有焦磷酸酶和磷酸二酯酶活性的非特异性酶。

Biochim Biophys Acta. 1978 Nov 10;527(1):70-85. doi: 10.1016/0005-2744(78)90257-7.

引用本文的文献

Cell Wall and Cytoplasmic Isozymes of Radish beta-Fructosidase Have Different N-Linked Oligosaccharides.萝卜β-果聚糖酶的细胞壁和细胞质同工酶具有不同的 N-连接寡糖。

Plant Physiol. 1986 Jan;80(1):27-33. doi: 10.1104/pp.80.1.27.

Parsley protoplasts retain differential responsiveness to u.v. light and fungal elicitor.欧芹原生质体保持对紫外线和真菌诱导子的差异响应性。

EMBO J. 1987 Sep;6(9):2551-6. doi: 10.1002/j.1460-2075.1987.tb02543.x.

Polypeptides of DNA-dependent RNA polymerase of spinach chloroplasts: characterization by antibody-linked polymerase assay and determination of sites of synthesis.菠菜叶绿体依赖 DNA 的 RNA 聚合酶的多肽：通过抗体连接聚合酶测定法的表征和合成部位的测定。

EMBO J. 1985 Jul;4(7):1661-6. doi: 10.1002/j.1460-2075.1985.tb03834.x.

Antibody-linked polymerase assay on protein blots: a novel method for identifying polymerases following SDS-polyacrylamide gel electrophoresis.蛋白质印迹上的抗体连接聚合酶测定：一种在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后鉴定聚合酶的新方法。

EMBO J. 1983;2(2):233-7. doi: 10.1002/j.1460-2075.1983.tb01411.x.

Identification of F11 fimbriae on chicken Escherichia coli strains.鸡源大肠杆菌菌株上F11菌毛的鉴定

Infect Immun. 1993 Mar;61(3):800-6. doi: 10.1128/iai.61.3.800-806.1993.

Human placenta: a convenient source of homologous material, for diagnosis and therapy of inborn errors of metabolism.

J Inherit Metab Dis. 1984;7(4):175-8. doi: 10.1007/BF01805606.

On the presence of two non-specific nucleotide-sugar-hydrolysing enzymes in rat liver.大鼠肝脏中两种非特异性核苷酸糖水解酶的存在

Biochem J. 1984 May 15;220(1):95-103. doi: 10.1042/bj2200095.

Synthesis and distribution of laminin-related polypeptides in early amphibian embryos.

Cell Tissue Res. 1986;246(1):45-51. doi: 10.1007/BF00218997.

Characterisation of an antiserum and development of an ELISA for glutathione peroxidase.

Vet Res Commun. 1986 Jul;10(4):269-81. doi: 10.1007/BF02213990.

Identification of the heat-labile hemolysin of Actinobacillus pleuropneumoniae serotype 1.胸膜肺炎放线杆菌1型耐热溶血素的鉴定

Can J Vet Res. 1989 Apr;53(2):251-4.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验