Ghiasuddin S M, Kawauchi S, Matsumura F, Doherty J D
Biochem Pharmacol. 1982 Apr 15;31(8):1483-9. doi: 10.1016/0006-2952(82)90370-7.
Efforts were made to understand the nature of the site of 1,1-bis-(p-chlorophenyl)-2,2,2-trichloroethane (DDT) inhibition of nerve ATPase. THe phospholipid content of nerve preparations from the walking leg of the lobster was reduced by treating them with phospholipase A, or with a chloroform-methanol mixture at -75 degrees. By these treatments the enzymes lost approximately 70 or 95% of their phospholipids and 50-80% of their Na,K- and Ca-ATPase activities. The lost ATPase activities could be partially restored by the addition of phospholipids, either the ones extracted from the lobster nerves or those from commercial sources. ATPase inhibition by DDT and permethrin was found to be highest in preparations where the phospholipids were removed by the above treatments, next highest with the untreated original enzyme, and least with the reconstituted ATPase regardless of the source of phospholipids used for reconstitution. This tendency was more pronounced in the case of Ca-ATPase. The effects of DDT and permethrin on inhibition of reconstituted Ca-ATPase were higher when the insecticide was first added to the protein portion and the enzyme was then reconstituted with the phospholipids, than when the same amount of insecticide was first added to the phospholipids which were then used for reconstitution.
人们致力于了解1,1-双（对氯苯基）-2,2,2-三氯乙烷（滴滴涕）对神经ATP酶抑制位点的性质。用磷脂酶A或在-75摄氏度下用氯仿-甲醇混合物处理龙虾步足的神经制剂，可降低其磷脂含量。通过这些处理，酶失去了大约70%或95%的磷脂以及50%-80%的钠钾-ATP酶和钙-ATP酶活性。添加磷脂后，失去的ATP酶活性可部分恢复，这些磷脂既可以是从龙虾神经中提取的，也可以是来自商业来源的。发现在上述处理去除磷脂的制剂中，滴滴涕和氯菊酯对ATP酶的抑制作用最强，其次是未处理的原始酶，而无论用于重构的磷脂来源如何，重构后的ATP酶受到的抑制作用最小。这种趋势在钙-ATP酶的情况下更为明显。当先将杀虫剂添加到蛋白质部分，然后用磷脂重构酶时，滴滴涕和氯菊酯对重构钙-ATP酶的抑制作用，比先将相同量的杀虫剂添加到用于重构的磷脂中时更高。
