Vodjdani G, Le Roscouet D, Horth M, Perucca-Lostanlen D, Gallien C, Doly J
Biochem Int. 1985 Mar;10(3):495-505.
A fraction enriched in interferon (IFN) mRNA was prepared from mouse C243-3 induced cells and was used for the construction of a cDNA library. Two plasmids were obtained after screening by differential colony hybridization and IFN mRNA hybridization-selection and translation. The nucleotide sequences of the cDNA inserts revealed that both were partial copies of IFN-beta mRNA. The cDNA 861 corresponds to the entire 3' nontranslated region of the mRNA while the cDNA 2939 consists of rearranged translated regions of IFN mRNA. A mechanism for the rearrangement events during cDNA synthesis is proposed. A chromosomal DNA fragment hybridizing to cDNA 2939 was identified by screening a mouse genomic library.
从小鼠C243 - 3诱导细胞中制备了富含干扰素（IFN）mRNA的组分，并用于构建cDNA文库。通过差异菌落杂交、IFN mRNA杂交选择和翻译筛选后获得了两个质粒。cDNA插入片段的核苷酸序列显示，两者均为IFN-β mRNA的部分拷贝。cDNA 861对应于mRNA的整个3'非翻译区，而cDNA 2939由IFN mRNA的重排翻译区组成。提出了cDNA合成过程中重排事件的机制。通过筛选小鼠基因组文库鉴定了与cDNA 2939杂交的染色体DNA片段。
